长链非编码RNA在心房颤动大鼠心房组织中的差异表达

Differential expression of long non-coding RNA in atrial tissue of atrial fibrillation rats

目的筛选心房颤动大鼠与正常大鼠心房组织长链非编码RNA(Lnc RNA)差异表达谱。方法选取基线水平相似的雄性野生型SD大鼠40只,应用随机数字表法分为对照组与心房颤动组,各20只。心房颤动组大鼠每天静脉注射心房颤动诱导药液0. 1 ml/100 g,连续给药7 d;对照组静脉注射0. 1 ml/100 g的0. 9%氯化钠注射液。心电图监测大鼠心房颤动情况。采用Lnc RNA基因芯片实验筛选2组大鼠左心房组织差异表达的Lnc RNA,并采用实时荧光定量聚合酶链反应法检测筛选出的Lnc RNA在2组大鼠心房组织中的实际表达情况。结果心房颤动组均造模成功。Lnc RNA基因芯片实验检测显示,与正常大鼠心房组织相比,心房颤动组大鼠心房组织Lnc RNA表达谱系发生显著变化,其中表达上调的Lnc RNA分别为TMEM252、USP18、CXCL10、OTOF与IFI27,表达下调的Lnc RNA分别为GRASP、LAMB3、PLAU与SEMG1。心房颤动组大鼠心房组织TMEM252、USP18、CXCL10、OTOF与IFI27表达水平均高于对照组[(4. 32±0. 54)比(0. 49±0. 13)、(16. 42±3. 46)比(2. 31±0. 51)、(7. 83±1. 32)比(1. 18±0. 33)、(25. 97±5. 13)比(4. 52±1. 92)、(10. 09±2. 12)比(1. 36±0. 63)],GRASP、LAMB3、PLAU与SEMG1表达水平均低于对照组[(0. 92±0. 33)比(2. 49±0. 82)、(1. 03±0. 41)比(5. 13±1. 12)、(2. 86±0. 42)比(14. 38±1. 93)、(1. 63±0. 44)比(10. 42±2. 19)],差异均有统计学意义(均P <0. 05),且趋势与芯片结果相符。结论心房颤动大鼠心房组织存在显著Lnc RNA差异表达谱变化。

Objective To explore the differential expression of long non-coding RNA(LncRNA) in atrial tissue between normal rats and atrial fibrillation rats. Methods Forty wild-type adult male SD rats were randomly assigned into control group and atrial fibrillation group with equal amount. The atrial fibrillation group had intravenous injection of 0.1 ml/100 g atrial fibrillation-inducing agents and the control group had intravenous injection of 0.1 ml/100 g saline for 7 days. Atrial fibrillation was detected by electrocardiogram. LncRNA microarray was performed to screen the differential expression. Fluorescence quantitative polymerase chain reaction was performed to verify the microarray data. Results Compared with the control group, the atrial fibrillation group showed a different LncRNA profile, including 5 up-regulated LncRNAs(TMEM252, USP18, CXCL10, OTOF, IFI27) and 4 down-regulated LncRNAs(GRASP, LAMB3, PLAU, SEMG1). Expression levels of TMEM252, USP18, CXCL10, OTOF and IFI27 in the atrial fibrillation group were significantly higher than those in the control group[(4.32±0.54) vs (0.49±0.13),(16.42±3.46) vs (2.31±0.51),(7.83±1.32) vs (1.18±0.33),(25.97±5.13) vs (4.52±1.92),(10.09±2.12) vs (1.36±0.63)];expressions of GRASP, LAMB3, PLAU and SEMG1 in the atrial fibrillation group were significantly lower than those in the control group[(0.92±0.33) vs (2.49±0.82),(1.03±0.41) vs (5.13±1.12),(2.86±0.42) vs (14.38±1.93),(1.63±0.44) vs (10.42±2.19)](all P<0.05). Conclusion LncRNA expression profile shows significant change in atrial tissue of rats suffering from atrial fibrillation.