骆驼尿液对小鼠酒精性急性肝损伤的预防作用的研究

Protective effect of camel urine on alcoholic acute liver injury in mice

本文旨在研究骆驼尿液对酒精引起的小鼠急性肝损伤的预防保护作用,观察骆驼尿液对酒精性肝损伤小鼠生长、肝指数、血清生化指标、炎症细胞因子释放及肝脏抗氧化能力及组织病理损伤的变化。SPF级雄性ICR小鼠40只按体重被随机分为5组,分别为空白对照组,酒精肝模型组,骆驼尿液低剂量组、骆驼尿液高剂量组、阳性药物对照组。骆驼尿液低剂量组与骆驼尿液高剂量组分别以250 mg/kg(按照体重)、500mg/kg(按照体重)骆驼尿液冻干粉的剂量进行灌胃,阳性药物组灌胃剂量为50 mg/kg水飞蓟素,灌胃体积为10 ml/kg,空白对照组及酒精肝模型组灌以等体积生理盐水。连续灌胃28 d,每天1次。28 d后,除空白对照组外,其余4组均灌胃17.7 m L/kg的50%乙醇(无水乙醇7 g/kg),建立急性酒精性肝损伤模型。禁食12 h后对小鼠进行称重,脱颈处死。测定血清中天门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活性与肿瘤坏死因子(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)含量,以及肝组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽酶(GSH-Px)和甘油三酯(TG)水平,并对肝脏进行组织病理检查。结果显示,与空白对照组相比,模型组小鼠ALT、AST、TNF-α、IL-1β、IL-6、TG水平均极显著增加(P<0.01),SOD、GSH-PX水平极显著下降(P<0.01),即造模成功。与酒精性肝损伤模型组相比,骆驼尿液高剂量组与阳性给药组ALT、AST、TNF-α、IL-1β、IL-6、TG水平均极显著降低(P<0.01),SOD、GSH-PX水平极显著增加(P<0.01)。上述试验结果表明,骆驼尿液对酒精所致急性肝损伤具有预防保护作用。

This paper aims to study the protective effect of camel urine on alcohol-induced acute liver injury in mice,and observe the growth,liver index,serum biochemical index,inflammatory cytokine release and liver antioxidant capacity of camel urine,and changes of histopathological damage in mice with alcoholic liver injury.40 SPF male ICR mice were randomly divided into 5 groups according to body weight,which were blank control group,model group,low-dose camel urine group,high-dose camel urine group and positive drug control group,respectively.The low-dose camel urine group and the high-dose camel urine group were intragastrically administered with 250 mg/kg and 500 mg/kg camel urine lyophilized powder according to body weight,respectively.The positive drug group was given a dose of 150 mg/kg silymarin according to body weight through administration by gavage,and the gavage volume was 10 m L/kg.The blank control group and the alcoholic liver model group were given an equal volume of normal saline.They were all given by gavage once a day for 28 days simultaneously.After 28 days,except for the blank control group,the other 4 groups were given 50% ethanol(17.7 m L/kg) to replicate the model of acute alcoholic liver disease.After fasting for 12 h,the mice were weighed and sacrificed by cervical dislocation.AST and ALT activities and levels of TNF-α,IL-1β,IL-6,and MDA,SOD,GSH-Px and TG levels in liver tissues were measured,and his topathological examination was performed on the liver.In result,the levels of AST,ALT,TNF-α,IL-1β,IL-6 and TG were significantly increased compared with the control group(P<0.01),and the level of SOD and GSH-PX were significantly decreased(P<0.01),which means the model is successful.Compared with the model group,the levels of AST,ALT,TNF-α,IL-1β,IL-6 and TG were significantly decreased(P<0.01),and the level of SOD and GSH-PX were significantly increased(P<0.01).The above-mentioned results showed that camel urine has a preventive and protective effect on alcohol-induced acute liver injury.