肌醇对小麦萌发期耐盐性的调节作用及生理机制分析

Regulatory effect and physiological mechanism analysis of inositol on salt tolerance of wheat germination

以小麦品种西农979为材料,确定了用于小麦盐胁迫的NaCl浓度为300mmol·L^-1,然后通过添加1、2、4、8mmol·L^-1的肌醇,观测在肌醇作用下小麦种子发芽率及丙二醛(MDA)含量。结果发现8mmol·L^-1外源肌醇可使受盐胁迫的小麦种子7d发芽率提高21.3%,MDA含量下降41.8%,MDA含量接近未受胁迫的小麦水平。同时,检测了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性,添加肌醇使盐胁迫下小麦SOD和CAT酶活性分别提高22.9%、15.0%,POD酶活性降低19.7%。研究表明,肌醇显著降低盐胁迫下小麦发芽期的丙二醛含量,对盐胁迫下小麦的损伤起到了一定的缓解作用。

Using Xinong 979 of wheat variety as the material,NaCl concentration for wheat salt stress was confirmed to be 300 mmol·L^-1.The inositol of 1,2,4 and 8 mmol·L^-1 were added to test the germination rate of wheat seeds and malondialdehyde(MDA)content under the action of inositol.The results showed that the exogenous inositol of 8 mmol·L^-1 could increase the germination rate by 21.3%and decrease the MDA content by 41.8%on 7 days of wheat seeds germination under salt stress.The MDA content was close to that of unstressed wheat.Meanwhile,the activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD)were also detected.The results showed that the SOD and CAT enzyme activities increased 22.9%and 15.0%,the POD enzyme activity decreased 19.7%by adding to the inositol for the wheat under the NaCl stress.The study indicated that the extra inositol could reduce the MDA content obviously and alleviate the damage of wheat under salt stress.