生防菌HFW217的鉴定及其对棉花枯萎病的防治效果

Identification and Control Effect on Cotton Fusarium wilt of Biocontrol Bacteria HFW217

【目的】研究生防菌HFW217的种类及其对棉花枯萎病的防治效果,为其生物防治提供材料。【方法】采用16SrDNA、rpoB基因序列和生理生化方法,对HFW217进行鉴定,通过温室和田间药效试验,测定HFW217对棉花枯萎病的防效。【结果】菌株HFW217为革兰氏阳性菌、呈短杆状、有芽孢,其菌体的形态呈短杆状,具有一定的耐盐碱性,16SrDNA和rpoB基因序列与解淀粉芽孢杆菌Bacillus amloliquefaciens具有较高的同源性,经鉴定该菌是解淀粉芽孢杆菌B.amloliquefaciens。温室和田间对棉花枯萎病防治效果分别达到77.62%和73.18%,防效明显高于多菌灵。【结论】菌株HFW217是解淀粉芽孢杆菌,该菌对棉花枯萎病的预防和治疗作用显著。

【Objective】 To clarify the types of biocontrol bacteria HFW217 and its control effects against cotton Fusarium wilt.【Method】 The 16S rDNA,rpoB gene sequence and physiological and biochemical method were adopted to identify HFW217,and the biocontrol effects of HFW217 were determined through greenhouse and field efficacy trials.【Result】 The analysis of salt tolerance showed that strain HFW217 had certain salt tolerance.The strain HFW217 microscopy was Gram-positive bacteria;It was short rod-shaped and spore-like,and its form wais short rod-like.16S rDNA and rpoB gene sequence had high homology with that of Bacillus amloliquefaciens homology.Identification results showed that biocontrol bacteria HFW217 was Bacillus amloliquefaciens;its preventive and control effects against cotton Fusarium wilt in the greenhouse reached 77.62% and 73.18%,respectively;Its control effects were significantly higher than that of carbendazim.【Conclusion】 The results showed that strain HFW217 was Bacillus amloliquefaciens,which had potential application in the biological control of Fusarium wilt of cotton.