雌激素对奶牛乳腺上皮细胞增殖及细胞周期变化规律的影响

Effects of estrogen on the proliferation and cell cycle of mammary gland epithelial cells in dairy cows

【目的】研究雌激素对乳腺上皮细胞增殖及细胞生长周期变化规律的影响。【方法】选取扩增生长状态良好的乳腺上皮细胞,添加0、50、100、200μmol/L雌激素孵育乳腺上皮细胞后,分别在0、12、24、48、72h后检测各组细胞增殖及细胞周期。【结果】培养12h时,50μmoL/L雌激素组的OD值显著高于对照组及100μmoL/L雌激素组(P<0.05),且极显著高于200μmoL/L雌激素添加组(P<0.01);培养至12h时,对照组及添加50μmoL/L雌激素组其G1期极显著高于其他两组(P<0.01),对照组S期极显著高于其他各组(P<0.01),添加100μmoL/L雌激素组其G2期极显著高于其他各组(P<0.01)。【结论】添加雌激素可以促进乳腺上皮细胞的增殖,且50μmoL/L、12h时雌激素促进乳腺上皮细胞的增殖效果最好。添加雌激素不影响乳腺上皮细胞在各时期时细胞生长的基本规律,各雌激素添加组在12h时的细胞周期由G1、S期向G2期转化较快。

【Objective】 To investigate the effects of estrogen on the proliferation and cell cycle of mammary epithelial cells.From November 2017 to January 2018,the experiment of estrogen on the proliferation and growth cycle of mammary epithelial cells was carried out in Xinjiang Herbivore Animal Nutrition Laboratory.【Method】 The mammary epithelial cells with good growth state were selected and the mammary epithelial cells were incubated with 0,50,100,200 μmol/L estrogen.The proliferation and cell cycle of each group were detected at 0,12,24,48 and 72 h respectively.Happening.【Result】 The results showed that the OD value of the 50 μmoL/L estrogen group was significantly higher than that of the control group and the 100 μmoL/L estrogen group ( P <0.05),and was significantly higher than the 200 μmoL/L estrogen addition group ( P <0.01).In the control group and the 50 μmoL/L estrogen group,the G1 phase was significantly higher than the other two groups ( P <0.01),and the S phase of the control group was significantly higher than the other groups ( P <0.01).The G2 phase of the 100 μmol L/L estrogen group was significantly higher than the other groups ( P <0.01).【Conclusion】 The results showed that the addition of estrogen can promote the proliferation of mammary epithelial cells,and estrogen promoted the proliferation of mammary epithelial cells at 50 μmol/L and 12 h.The addition of estrogen did not affect the basic regularity of cell growth in mammary epithelial cells at various stages.The cell cycle of each estrogen-added group changed from G1 to S to G2 at 12 h.