TNIP1基因沉默对系统性红斑狼疮患者B细胞上BCR、CD40基因表达的影响

Effect of TNIP1 gene silence on the expression of BCR and CD40 gene in B cells of patients with systemic lupus erythematosus

目的探讨TNIP1基因沉默对系统性红斑狼疮(SLE)患者B细胞上BCR和CD40基因表达的影响.方法收集2018年3月武汉大学医院接收的20例SLE患者和20例健康体检者的血液标本,以慢病毒介导TNIP1基因沉默为切入点,采用实时荧光定量PCR方法检测细胞中的TNIP1基因中mRNA的表达水平以及BCR、CD40的表达水平.结果TNIP1基因在SLE患者中的表达量较高,干扰B细胞中TNIP1基因可导致B细胞中BCR和CD40表达下调.结论TNIP1、BCR和CD40基因在B细胞中的表达具有一致的表达趋势,提示TNIP1、BCR、CD40有一定的调控关系.

Objective To explore the effect of TNIP1 gene silence on the expression of BCR and CD40 gene in B cells of patients with systemic lupus erythematosus (SLE). Methods Blood samples from 20 SLE patients and 20 healthy subjects received in Wuhan University Hospital during March 2018 were collected. Lenti viral-mediated TNIP1 gene silence was used as an entry point. Real-time quantitative PCR was used to detect mRNA expression level as well as expression levels of BCR and CD40 in TNIP1 gene in cells. Results The expression level of TNIP1 gene was higher in SLE patients, and interference with TNIP1 gene in B cells could lead to down-regulation of BCR and CD40 expression in B cells. Conclusion The expression of TNIP1, BCR and CD40 genes in B cells show a consistent trend, suggesting that TNIP1, BCR and CD40 have a certain regulatory relationship.