吊兰属植物Chlorophytum tuberosum的化学成分及其对血小板聚集的作用

Study on the chemical constituents and effect on platelet aggregation of Chlorophytum tuberosum

[目的]研究Chlorophytum tuberosum50%乙醇提取物中的化学成分及其对血小板聚集的作用。[方法]运用各种柱色谱及制备型高效液相等对化学成分进行分离纯化,综合运用NMR、LC-MS等波谱技术鉴定化合物结构;以ADP为激动剂,阿司匹林为阳性对照,初步测定了不同极性洗脱物及部分化合物对血小板聚集的影响。[结果]共分离得到11个化学成分,分别为:新替告皂苷元(1)、(25S)-曼诺皂苷元(2)、新海柯皂苷元(3)、新海柯皂苷元-3-O-β-D-葡萄糖-(1→2)-[β-D-葡萄糖-(1→3)]-β-D-葡萄糖-(1→4)-β-D-半乳糖苷(4)、新吉托皂苷元-3-O-β-D-葡萄糖-(1→2)-[β-D-葡萄糖-(1→3)]-β-D-葡萄糖-(1→4)-β-D-半乳糖苷(5)、新吉托皂苷元-3-O-β-D-葡萄糖-(1→2)-[β-D-葡萄糖-(1→4)]-β-D-葡萄糖-(1→4)-β-D-半乳糖苷(6)、新吉托皂苷元-3-O-β-D-葡萄糖-(1→2)-[β-D-木糖-(1→3)]-β-D-葡萄糖-(1→4)-β-D-半乳糖苷(7)、大叶吊兰苷C(8)、大叶吊兰苷E(9)、β-谷甾醇(10)和胡萝卜苷(11)。[结论]化合物1为吊兰属首次分离,化合物2为百合科首次分离,其他化合物均为首次从该植物中分离得到;70%乙醇洗脱物及化合物6、7有诱导血小板聚集的活性,而95%乙醇洗脱物有抑制血小板聚集的活性。

[Objective]To investigate the chemical constituents from 50%(v/v)ethanol extract of C.tuberosum and their effect of platelet aggregation activity.[Methods]The chemical constituents were isolated and purified through various columns chromatography and PHPLC.Their structures were identified by NMR and LC-MS spectra.The different polar parts and compounds isolated from C.tuberosum were tested by in vitro anti-platelets aggregation assay.[Results]Eleven compounds were isolated and identified as neogitogenin(1),(25S)-manogenin(2),neohecogenin(3),neohecogenin-3-O-β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(4),neogitogenin-3-O-β-D-glucopy ranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(5),neogitogenin-3-O-β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(6),neogitogenin-3-O-β-D-xylopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(7),neogitogenin-3-O-β-D-xylopyranosyl(1→3)-[α-L-arabinopyranosyl(1→2)]-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-galactopyranoside(8),26-O-β-D-glucopyranosyl-22-hydroxy-25(S)-5α-furostan-12-oxo-3β,26-diol-3-O-β-D-xylopyranosyl(1→3)[α-L-arabinopyranosyl(1→2)]-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-galactopyranoside(9),β-sitosterol(10),daucosterol(11).[Conclusion]Compound 2 was isolated from the Liliaceae for the first time,Compound 1 was isolated from the genus Chlorophytum for the first time and the other compounds were isolated from this species for the first time.The platelet aggregation activity of the different polar parts and compounds were tested by in vitro anti-platelets aggregation,The result indicated that the 95%part had the potent bioactivity against ADP-induced platelet aggregation,however the 70%part had the bioactivity of inducing platelet aggregation.