摘要
目的建立快速、灵敏、高效的精氨酸双糖苷的测定方法并研究精氨酸双糖苷在大鼠体内的药动学。方法以乌苯美司作为内标,采用LC-MS/MS检测方法测定血浆中精氨酸双糖苷浓度,利用DAS2.0软件计算药动学相关参数,并分析方法的可靠性。结果空白血浆对检测物质不产生干扰,在20～20 000 ng/mL符合线性关系,方法学中RSD以及RE值均小于15%。结论采用LC-MS/MS方法检测精氨酸双糖苷方法合理可靠,精氨酸双糖苷的绝对生物利用度为(4.50±3.75)%。
Objective To develop a quick, specific and high performance method for determination of Arginyl-fructosyl-glucose(AFG) and to investigate pharmacokinetics of AFG in rats in vivo. Methods Bestatin is used as an internal standard, the concentration of AFG in plasma was determined by LC-MS/MS. The DAS 2.0 software was adopted to calculate bioavailability of AFG. Results Blank plasma does not interfere with the test substance, there is a significant correlation in the range of 20 ng/mL to20 000 ng/mL, and the RSD and RE values are less than 15% in the method. Conclusion The average relative bioavailability of AFG was(4.50 ± 3.75)%, The measured method of AFG by LC-MS/MS is reasonable and reliable.
作者
毕筱婷
王长远
李珂珂
霍晓奎
刘克辛
弓晓杰
BI Xiaoting;WANG Changyuan;LI keke;HUO Xiaokui;LIU Kexin;GONG Xiaojie(Medical college of Dalian University, Dalian 116622, China;Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian 116044, China;College of Life Science, Dalian Nationalities University, Dalian 116600, China)
出处
《药物评价研究》
CAS
2019年第6期1141-1145,共5页
Drug Evaluation Research
基金
辽宁省高等学校创新团队支持计划(LT2016017)
大连市科技创新基金(2018J12SN062)
关键词
精氨酸双糖苷
精密度
准确度
药动学
方法学
Arginyl-fructosyl-glucose
relative standard deviation
relative error
pharmacokinetic
methodology
