响应面法优化超声辅助提取贝母多酚的工艺

Optimization of ultrasonic assisted extraction of polyphenols from Fritillaria by Response surface methodology

目的利用响应面法优化超声-辅助提取贝母多酚的工艺条件。方法在单因素实验的基础上,以乙醇浓度、料液比、提取温度对贝母多酚提取率的影响为自变量,以没食子酸为对照,以多酚提取率为响应值,应用中心组合设计实验方法,研究各自变量及其交互作用对多酚提取率的影响,建立二次多项回归方程的数学模型。结果实验表明:料液比对贝母多酚提取率的影响比较显著。结论最终优选的超声波提取贝母多酚工艺为:62%乙醇、料液比为1∶27、提取温度61℃,在此条件下贝母多酚的实际提取率为7.36%,与理论值7.43%相差0.07%。说明该工艺可用于贝母中多酚的提取,且方法稳定可靠。

OBJECTIVE To optimize the ultrasonic assisted extraction process of Fritillaria polyphenols by Response surface methodology.METHODS On the basis of single factor experiment,using ethanol concentration,material to liquid ratio,and extraction temperature as independent variables,with gallic acid as standard,the yield of polyphenols as the response value,the application of central composite experimental design method,to study the interactions between these variables and their influence on the yield of polyphenols.The mathematical model of two multinomial regression equation was established.RESULTS The experimental results showed that material to liquid ratio on the extraction rate of Fritillaria polyphenols had obvious effects.CONCLUSION The final optimization of ultrasonic extraction of Fritillaria polyphenols process is:Ethanol concentration of 62%,the ratio of liquid of 1∶27,extraction temperature of 61℃,the actual extraction rate of polyphenols in Fritillary was 7.36%,compared with 0.07%of the theoretical value 7.43%.It indicates that the process can be used to extract polyphenol from Fritillary and the method is stable and reliable.