多巴胺D1受体在小鼠大脑皮层的空间分布研究

A systematic morphological study of dopamine receptor 1 distribution in cerebral cortex of mice

目的:利用多巴胺受体1(D1Rs)荧光转基因小鼠系统探讨D1Rs在小鼠大脑皮层的空间分布特征和相对表达模式。方法:使用成年Drd1-tdTomato转基因小鼠,制备冠状全脑切片,采用DAPI衬染细胞核,在共聚焦显微镜下观察D1Rs的分布,使用Imaris软件荧光阈值点自动计数算法实现细胞计数。结果:D1Rs在大多数大脑皮层区域内都有表达,但总体比例较低,这些区域包括眶皮层、岛叶皮层、扣带皮层、视皮层、听觉皮层、运动皮层、躯体感觉皮层、外嗅皮层和梨状皮层等。同时我们发现D1Rs在大脑皮层中主要集中在浅层(1/2层)和深层(5/6层)。结论:不同大脑皮层区域D1Rs分布模式不同。

Objective: Using dopamine receptors D1(D1Rs) transgenic mice to explore the distribution and relative expression patterns of D1Rs in different cortical regions systematically. Methods: Adult Drdl-tdTomato transgenic mice were used for preparing coronal brain slices containing the whole brain,DAPI was used to stain nuclei. Observations were carried out under the confocal microscope. And the expression patterns of D1Rs were determined by counting the number of cells achieved by automatic spots algorithm based on the fluorescent threshold using the Imaris software.Results: D1Rs expressed in most cerebral cortex regions but totally in a relatively low proportion,such as orbital cortex,insular cortex,cingulate cortex,visual cortex,auditory cortex,motor cortex,somatosensory cortex,piriform cortex and ectorhinal cortex. And we found that the expression of D1Rs in cerebral cortex was mostly in the superficial(layers1/2) and deep layers(layers 5/6). Conclusion: There is a variable distribution pattern of D1Rs in cerebral cortex regions.