9个基因在桑树硬枝扦插生根过程中的表达特性分析

Expression Characteristics of Nine Genes in Rooting Process of Mulberry Hardwood Cutting

桑树扦插生根涉及复杂的生物学过程,受多种内源激素和环境因素的调节。为发掘桑树扦插生根相关基因,为桑树扦插不定根的形成和调节提供分子理论依据,采用桑品种育711号1年生硬枝为插穗,分别以传统愈伤组织生根技术和新的可诱导皮部生根的技术进行处理,以清水处理为对照,利用qRT-PCR技术检测PPO1、PPO2、PPO3、HSP83A、ILL5、GH3.1、SAUR2、Cacybp、ANT1 9个基因在愈伤组织生根和皮部生根2种生根形式生根过程中的表达变化。结果显示在扦插生根的不同发育阶段,9个基因在皮部生根处理组的表达量均明显高于愈伤组织生根处理组和对照组,且各个基因表达量在不同形式的生根过程中表现出不同的变化趋势,初步推测这些基因在不定根的形成中均发挥了重要作用。研究结果将丰富桑树甚至其他林木扦插生根机制的内容,并可为进一步开发高效实用的桑树扦插技术奠定良好的理论基础。

Rooting process of mulberry cutting is a complex biological process affected by various endogenous hormones and environmental factors. In order to explore the rooting related genes of mulberry cuttings and provide molecular basis for the formation and regulation of adventitious roots, one-year hardwood branches of a mulberry variety named Yu 711 were selected and treated with traditional callus rooting technique and novel inducible cortex rooting technique. Branches treated with water were used as control. Expression levels of 9 genes including PPO1, PPO2, PPO3, HSP83A, ILL5,GH3.1, SAUR2, Cacybp and ANT1 were detected by qRT-PCR to identify the changes of expression level in the process of callus rooting and cortex rooting. Results showed that the expression levels of 9 genes in cortex rooting group were significantly higher than those in callus rooting group and the control group, and expression levels of different genes showed different trends in different rooting types. It is preliminarily speculated that these genes play important roles in the formation of adventitious roots. The results will facilitate further researches on rooting mechanism of cuttings in mulberry and other trees, and lay a profound theoretical foundation for the promotion of efficient and practical mulberry cutting skills.