丝素蛋白-氧化石墨烯多孔微球支架的制备及性能测试

Preparation and Property Analysis of Silk Fibroin-graphene Oxide Porous Microsphere Scaffold

为了拓展丝素蛋白(SF)在组织工程等生物医学领域的应用,采用微乳液-冷冻相分离技术制备丝素蛋白-氧化石墨烯(SF-GO)复合多孔微球支架,并通过傅里叶变换红外光谱(FTIR)、拉曼光谱(Raman)、扫描电子显微镜(SEM)及热重分析(DTG-TG)等方法对SF-GO复合支架的化学结构和形貌等理化性质进行分析表征。结果表明,宏观上SF微球支架和SF-GO复合微球支架均呈现为表面规则平滑的球形且粒径均匀,但SF-GO微球支架比SF微球支架有更大的微孔结构;加入氧化石墨烯后,微球支架的热稳定性能得到明显改善。体外细胞培养实验表明该微球支架有良好的生物相容性,且SF-GO复合微球支架比普通SF微球支架更能够促进骨髓间充质干细胞的黏附和增殖。

In order to promote silk fibroin(SF) application in biomedical field like tissue engineering, silk fibroin-graphene oxide(SF-GO) composite porous microspheres scaffolds were prepared in this study by micro-emulsion-freezing phase separation technique, and the physicochemical properties of SF-GO composite microsphere scaffolds were characterized by Fourier transform infrared spectroscopy(FTIR), Raman spectroscopy(Raman), scanning electron microscopy(SEM) and thermo gravimetric analysis(DTG-TG). Results showed that SF-GO composite microsphere scaffold had bigger microporous structure than SF microsphere scaffold, though SF microsphere scaffold and SF-GO composite microsphere scaffold had uniform macroscopic microspheres with smooth surface. The thermal stability of the microsphere scaffolds was also significantly improved with the addition of GO. Moreover, in vitro cell culture experiment indicated that SF-GO microsphere scaffolds showed outstanding biocompatibility, and human mesenchymal stem cells on SF-GO composite microsphere showed stronger ability of adhesion and proliferation than that on SF microsphere.