荧光碳点负载阿霉素载药体系在肝癌治疗中的应用

Application of Fluorescent Carbon Dot-Loaded Doxorubicin Drug Delivery System in the Treatment of Liver Cancer

以荧光碳点(CD)为载体、阿霉素(DOX)为抗肿瘤药物,通过静电吸附作用构建了一种pH响应型的碳点负载阿霉素(CD-DOX)载药体系。应用紫外可见吸收法、二甲基噻唑蓝法和荧光显微成像法对碳点负载阿霉素载药体系的性能进行了检测。实验结果表明:在pH值为7.4的环境中,当碳点与阿霉素的质量比为1时,得到的碳点负载阿霉素载药体系的药物负载率最大。碳点负载阿霉素载药体系具有敏感的pH响应性,它对人体肝癌细胞生长的抑制作用约是其对人体正常肝细胞抑制作用的1.7倍。同时,碳点负载阿霉素载药体系也能够长时间地实时监测细胞状态和追踪药物释放过程。因此,该载药体系的性能良好,有望应用于肝癌的治疗及可视化的药物追踪中。

A pH-responsive drug delivery system of carbon dot-loaded doxorubicin (CD-DOX) was constructed by electrostatic adsorption using fluorescent carbon dot (CD) as carrier and doxorubicin (DOX) as antitumor drug? The performance tests of the CD-DOX drug delivery system were carried out using ultraviolet-visible absorption,(dimethyl-2-thiazolyl) MTT and fluorescence microscopy methods? The experimental results show that in the environment of pH = 7. 4, when the mass ratio of CD and DOX is 1, the drug loading efficiency of the obtained CDDOX drug delivery system is the largest. The CD-DOX drug delivery system has sensitive pH responsiveness, and the inhibitory effect of which on the growth of the human liver cancer cell is about 1.7 times than that on the growth of the normal human liver cell. Meanwhile, the CD-DOX drug delivery system is also capable of monitoring the state of cells and tracking drug release process in real time for a long period of time. Therefore, the drug delivery system has good performance and is expected to be applied in the treatment of liver cancer and visualizing trace of the drug.