丙型肝炎病毒E2蛋白的生物信息学分析

Sequence characterization of E2 protein in hepatitis C virus

目的探讨研究丙型肝炎病毒E2蛋白的生物学特征。方法从NCBI数据库中获取丙型肝炎病毒E2蛋白氨基酸序列,利用在线分析软件分析其生物学特征,包括分子式、半衰期、疏水性、跨膜区、信号肽,磷酸化位点、二级结构特征等,通过综合分析丙型肝炎病毒E2蛋白的各类性质,得到最佳抗原表位区域,并对丙型肝炎病毒E2蛋白氨基酸序列预测结果进行了比对分析。结果丙肝病毒E2蛋白由344个氨基酸组成,分子式为C1696H2543N463O490S21,不稳定系数为35.6,理论等电点为7.2,总体平均亲水性为-0.131,为稳定性亲水蛋白质,E2蛋白二级结构中主要成分为无规则卷曲(占比43.6%)。该蛋白无信号肽且无跨膜区,约存在50个磷酸化位点,最佳抗原区域位于95-100氨基酸位置。结论生物信息学方法预测丙型肝炎病毒E2蛋白属于稳定性亲水蛋白,含有抗原表位区域,可为丙型肝炎表位疫苗的研制提供参考依据。

Objective Analyzing sequence characteristics of E2 protein in Hepatitis C Virus(HCV)were studied via bioinformatics tools.Methods The hepatitis C virus E2 protein amino acid sequence was obtained from the National Center for Biotechnology Information(NCBI)database.The online analysis software was used to analyze and study the sequence characteristics of hepatitis C virus E2 protein.The basic information analysis included molecular formula,half-life,hydrophobicity,transmembrane region,and signal peptide.Functional information analysis included phosphorylation sites and secondary structure characteristics.Finally,a comprehensive analysis of the various properties of the hepatitis C virus E2 protein was done to obtain the best epitope region,and the analysis of the above-mentioned prediction results of the amino acid sequence characteristics of the hepatitis C virus E2 protein were compared.Results E2 protein in Hepatitis C Virus consists of 344 amino acids,its molecular formula is C1696 H2543 N463 O490 S21,and the instability coefficient is 35.6.The protein is predicted to be a stable protein,the theoretical isoelectric point is 7.20,and the total average hydrophilicity is-0.131,which denotes that the E2 protein is a hydrophilic protein.The main component of the secondary structure is irregularly coiled(43.6%),the protein has no signal peptide,and no transmembrane region,50 phosphorylation sites.Conclusion Best antigenic epitope formation area was determined via a comprehensive analysis of the availability of E2 protein in HCV,βturn angle,antigenicity and hydrophobicity,etc.The study of E2 protein in HCV provides reference for the development of an epitope vaccine against HCV.