SF/SA/HBG纤维支架材料的构建及体外生物矿化

Construction of SF/SA/HBG fiber scaffold materials and in-vitro biomineralization

由静电纺丝技术纺制的纤维支架材料能够提供大的比表面积及较高的孔隙率。以甲酸为溶剂,丝素蛋白(Silk fibroin,SF)和海藻酸钠(Sodium alginate,SA)为基体材料,并加入中空生物活性玻璃(Hollow bioactive glass,HBG),复合成体外生物活性较好的生物支架材料;通过体外生物矿化可以加速羟基磷灰石(Hydroxyapatite,HAp)的沉积及生长。经过一系列测试分析,结果显示:通过静电纺丝制备出SF/SA/HBG纤维复合膜,其平均直径分布在200~300 nm;经过乙醇处理后,纤维表面发生溶胀,直径变粗,平均直径分布在230~380 nm;进行体外生物矿化后,在纤维表面形成HAp,SF/SA/HBG纤维复合支架材料具有良好的生物活性。

The fiber scaffold material spun by electrospinning technology can provide large specific surface area and high porosity.In this paper,the biological scaffold material with good in-vitro biological activity was synthesized by using formic acid as the solvent,using silk fibroin(SF)and sodium alginate(SA)as matrix materials,and adding hollow bioactive glass(HBG).The deposition and growth of hydroxyapatite(HAp)could be accelerated through in-vitro biomineralization.After a series of tests and analysis,the results showed that the average diameter of the SF/SA/HBG fiber composite membrane prepared by electrospinning was between 200 nm and 300 nm.After ethanol treatment,the surface of the fiber swelled and the diameter became thick and the average diameter was distributed within is 230~380 nm.After in vitro biomineralization,hydroxyapatite was formed on the fiber surface,indicating that the SF/SA/HBG fiber composite scaffold material has good biological activity.