急性心肌梗死患者血浆中MEG3和UCA1的表达水平及临床意义

Plasma expression levels of MEG3 and UCA1 in patients with acute myocardial infarction and their clinical significance

目的:探讨急性心肌梗死(AMI)患者血浆中人类母系表达基因3(MEG3)和尿路上皮癌抗原1(UCA1)表达水平及其临床意义。方法:选择我院收治的90例AMI患者为AMI组,同时期50例在我院体检健康者为健康对照组,观察并比较健康对照组及AMI组发病后不同时间点(1h、3h、6h、12h)血浆MEG3、UCA1表达及血清肌酸激酶同工酶(CK-MB)和心肌肌钙蛋白I(cTnI)水平变化,采用Spearman相关分析其相关性。结果:与健康对照组比较,AMI患者发病1h后血浆MEG3[(0.002±0.001)比0.017±0.003)]表达显著升高,UCA1[(0.027±0.005)比(0.017±0.002)]表达显著降低(P均=0.001);3h后AMI患者血清CK-MB[(20.01±3.05)IU/L比(32.10±4.40)IU/L]、cTnI[(1.01±0.87)ng/L比(2.10±0.91)ng/L]水平均显著升高(P均=0.001)。Spearman相关分析显示:AMI患者血浆MEG3表达与血清CK-MB、cTnI水平呈显著正相关(r=0.351、0.368,P均<0.05);而血浆UCA1表达与血清CK-MB、cTnI水平呈显著负相关(r=-0.416、-0.425,P均<0.01)。结论:急性心肌梗死发作早期患者血浆中人类母系表达基因-3显著升高,尿路上皮癌抗原1显著降低,两者与CK-MB、cTnI水平有显著相关性,可作为急性心梗早期确诊的新型标志物。

Objective:To explore plasma expression levels of human maternal expression gene 3 (MEG3) and urothelial carcinoma antigen 1 (UCA1) in patients with acute myocardial infarction (AMI) and their clinical significance. Methods:A total of 90 AMI patients treated in our hospital were enrolled as AMI group, and 50 healthy subjects undergoing physical examination in our hospital simultaneously were treated as healthy control group. Plasma expressions of MEG3 and UCA1, serum levels of creatine kinase isoenzyme MB (CK-MB) and cardiac troponin I (cTnI) were observed and compared between healthy control group and AMI group on 1h, 3h, 6h and 12h after onset. Spearman correlation analysis was used to analyze their correlation. Results:Compared with healthy control group, there was significant rise in plasma MEG3 expression [(0.002±0.001) vs. 0.017±0.003)], and significant reduction in UCA1 expression [(0.027±0.005) vs.(0.017±0.002)]in AMI group on 1h after onset, P =0.001 both;after 3h, there were significant rise in serum levels of CK-MB[(20.01±3.05) IU/L vs.((32.10±4.40) IU/L]and cTnI[(1.01±0.87) ng/L vs.(2.10±0.91) ng/L]in AMI group, P =0.001 all. Spearman correlation analysis indicated that in plasma MEG3 expression was significant positively correlated with serum CK-MB and cTnI levels ( r =0.351, 0.368, P <0.05 both), and plasma UCA1 expression was significant inversely correlated with serum levels of CK-MB and cTnI ( r =-0.416,-0.425, P <0.01 both) AMI patients. Conclusion:Plasma MEG3 level significant rises, and UCA1 level significantly reduces in AMI patients during early onset period. Both are significantly correlated with CK-MB and cTnI levels, which may be used as new markers diagnosing early AMI.