药对知母-黄柏对胰岛素抵抗的改善作用

The improving effect of Zhimu-Huangbai herb pair on insulin resistance

目的 研究药对知母-黄柏(ZB)对胰岛素抵抗(IR)的改善作用。方法采用高脂高糖饲料联合链脲佐菌素建立2型糖尿病大鼠模型。随机分为正常组、模型组、二甲双胍组、知柏高、低剂量组(ZB 4.5、1.5 g·kg -1 ),连续灌胃给药4周。检测体质量、空腹血糖(FBG)、血清胰岛素水平(FINS)及甘油三脂含量(TG),计算胰岛素敏感指数(ISI)和胰岛素抵抗指数(Homa-IR)。另以棕榈酸和油酸联合诱导HepG2细胞产生IR。分为正常组、模型组、二甲双胍组、知柏高、中、低剂量组(ZB384、192、96 mg·L^-1 ),各组给药24 h后检测糖耗量及TG含量。结果药对知母-黄柏能明显降低糖尿病大鼠的FBG、FINS、TG含量( P <0.05)及Homa-IR( P <0.01),提高ISI( P <0.05),并能明显降低HepG2-IR细胞的TG含量( P <0.05),提升糖耗量( P <0.05)。结论药对知母-黄柏能提升胰岛素敏感性,促进糖脂代谢,对胰岛素抵抗具有改善作用。

Aim To explore the effects of Zhimu-Huangbai herb pair (ZB) on insulin resistance.Methods The rat model of experimental T2DM was induced by intraperitoneal injection of streptozotocin after feeding with high fat and sucrose forage.Rats with T2DM were randomly divided into normal group, model group, metformin group and high-, low-dose ZB group(4.5,1.5 g·kg^-1 ), with corresponding drugs administered orally by gastric gavage once a day for four weeks.The body weight, fasting blood glucose(FBG), serum insulin level(FINS) and triglyceride content(TG) were measured, and the insulin sensitivity index(ISI) and insulin resistance index(Homa-IR) were calculated.The HepG2 cells were treated with palmitate and oleate to induce insulin resistance, then divided into six groups: control group, model group, metformin group and high-, middle-, low-dose ZB group(384,192, 96 mg·L^-1 ).Glucose consumption and TG content were assayed24 h after administration.Results ZB not only markedly lowered the level of FBG, FINS, TG content( P <0.05) and insulin resistance index( P <0.01), increased insulin sensitivity index( P <0.05) in rats with T2DM, but also significantly decreased TG content( P <0.05) and increased glucose consumption( P <0.05) in HepG2 cell model with insulin resistance.Conclusions ZB can promote glucolipid metabolism and improve the insulin resistance by increasing insulin sensitivity.