年龄因素对牙周膜干细胞多向分化能力的影响

The effect of aging on the multi-directional differentiation ability of human periodontal ligament stem cells

目的:探讨年龄因素对人牙周膜干细胞(PDLSCs)多向分化能力的影响。方法:选取因阻生需要拔除的具有完整牙根的第三磨牙共14颗,根据患者年龄分为青少年组(16~19岁)和中老年组(38~50岁),分别刮取牙周膜组织,分离培养获得PDLSCs,用流式细胞术分析干细胞表面标记物;通过体外成骨、成脂诱导培养,比较两组PDLSCs的多向分化能力。结果:经过流式细胞术检测,两组PDLSCs均具有间充质干细胞的特性,但中老年组PDLSCs的成骨、成脂分化能力较青少年组明显减弱(P<0.05)。结论:PDLSCs的多向分化能力随年龄的增加而降低。

Objective: To investigate the effect of aging on the multi-directional differentiation ability of human periodontal ligament stem cells (PDLSCs). Methods: 14 healthy third molars were collected with complete roots in clinic and divided into two groups according to the donors’ ages: the younger group (donor age at 16-19 years) and the older group (donor age at 38-50 years). The periodontal ligament tissues were obtained and PDLSCs were isolated and cultured. Flow cytometry analysis was used to identify the phenotypes of PDLSCs. To compare the multi-directional differentiation ability of PDLSCs, cells were subjected to osteogenic and adipogenic induction in vitro respectively. Results: Flow cytometry analysis revealed that PDLSCs in both groups had the characteristics of mesenchymal stem cells. And the multi-directional differentiation ability of PDLSCs in the older group was significantly decreased compared with that in the younger group( P <0.05). Conclusions: The multi-directional differentiation ability of PDLSCs decreased with age.