西藏绒山羊的微卫星多态性与部分经济性状的关联分析

Correlation between Microsatellite Polymorphism and Economic Traits of Tibetan Cashmere Goat

为了解微卫星位点在西藏绒山羊群体中的多态性与经济性状的关联性,试验采用PCR-SSCP技术对340只西藏绒山羊的10个微卫星位点进行多态性分析,并采用Duncan氏法进行多重比较,与体长、体高、胸围、羊绒长度、羊毛长度的关联性进行分析。结果表明,除位点BM6438无多态性外,其他9个位点都具有多态性,基因型都在5个以上。与经济性状进行关联性分析发现在体长性状中,位点BM3033的基因型6显著高于其他基因型个体(P<0.05);在体高性状中,位点BMS1248的基因型1和位点BM3033的基因型6显著高于其他基因型;在胸围性状中,位点BMS1248的基因型2和位点BM3033的基因型6显著高于其他基因型个体(P<0.05);在绒长性状中,位点BMS1788的基因型3显著高于其他基因型个体(P<0.05);在毛长性状中,位点BM3413的基因型1和基因型2显著高于其他基因型个体(P<0.05)。本研究结果可为西藏绒山羊分子标记辅助选择提供参考依据。

In order to investigate the feasibility of microsatellite loci to be selected as genetic markers of growth indexes for Tibetan Cashmere goat population,the polymorphism of 10 microsatellite markers were detected by PCR-SSCP and DNA sequencing technology in Tibetan Cashmere goat,analyzed andcorrelation between microsatellite markers and economic traits by Duncan’s method for multiple comparisons were conducted. The results showed that loci microsatellite BM6438 was non-polymorphism loci,among the ten microsatellite markers,the other 9 loci were polymorphic,and the genotypes were more than 5 in Tibetan Cashmere goat. The correlation analysis results showed that as the body length genotype 6 of BM3033 was significantly higher than those of other genotypes( P < 0. 05);as for body height genotype 1 of BMS1248 and genotype 6 of BMS3033 was significantly higher than of other genotype( P < 0. 05);as for chest circumference,genotype 2 of BMS1248 and genotype 6 of BMS3033 were significantly higher than of other genotype( P < 0. 05);as for Cashmere length,genotypes 3 of BMS1788 were significantly higher than those of other genotype( P < 0. 05);as for goat hair length,genotypes 1 and 2 of BM3413 were significantly higher than of other genotype( P < 0. 05). The results of this study provide a reference for genetic markers of growth indexes for Tibetan Cashmere goat population.