游泳运动可抑制HCFD诱导肥胖大鼠模型凝血功能异常

Swimming Could Inhibit the Dysfunction of Blood Coagulation in Obese Rats Induced by HCFD

目的明确游泳运动是否可抑制高碳水化合物和脂肪饮食(HCFD)诱导肥胖大鼠凝血功能异常。方法40只雄性Wistar大鼠被随机分为4组,对照组、对照+游泳组、HCFD组和HCFD+游泳组。各组大鼠均饲养15周,其中对照组大鼠用标准实验室饲料喂养15周;HCFD组大鼠HCFD饲养15周。游泳组大鼠在第11周到第15周进行游泳训练。然后分别对各组大鼠炎性标志物和凝血相关指标等进行检测。结果HCFD导致大鼠血糖、IR、血脂异常和炎性生物标志物显著增高。此外,HCFD显著增加大鼠凝血和血栓形成生物标志物,如纤维蛋白原、纤溶酶原激活剂抑制物-1、凝血酶原时间、活化部分凝血活酶时间、凝血和出血时间以及血小板聚集等,而游泳运动可抑制HCFD大鼠上述指标的增加。结论在肥胖和IR大鼠模型中,通过游泳运动可以改善凝血功能异常。

Objective To determine whether swim exercise can inhibit high carbohydrate and fat diet ( HCFD)-induced biomarkers of coagulation. Methods 40 male Wistar rats were randomly divided into 4 groups: control group,control + swimming group,HCFD group,and HCFD + swimming group. Rats of control group were fed with standard laboratory chow for 15 weeks,and rats of HCFD group were fed HCFD for 15 weeks. Swim exercise-‘treated' rats started swim exercise training from the 11th week until being sacrificed,on week 15. Then inflammatory markers and coagulation related indicators were detected in each group. Results HCFD caused a significant increase in blood glucose,insulin resistance ( IR),lipidemia, and inflammatory biomarkers. In addition,HCFD significantly modulated coagulation and thrombosis biomarkers;fibrinogen, plasminogen activator inhibitor-1,prothrombin time,activated partial thromboplastin time,blood clotting and bleeding time, and platelet aggregation that was effectively inhibited by swimming exercises. Conclusions In an animal model of obesity and IR,there is a significant change in hemostasis,which is ameliorated by swim exercise.