陆地棉14-3-3基因家族的鉴定及逆境胁迫应答

Identification and Stress Response of 14-3-3 Gene Family in Upland Cotton(Gossypium hirsutum)

为了揭示棉花14-3-3家族的功能,本研究在陆地棉里鉴定了31个14-3-3蛋白,分析了它们的保守结构域、系统进化、理化性质、基因结构、保守motif以及基因的组织表达模式和逆境表达模式。结果显示,陆地棉31个14-3-3蛋白都有9个反向平行的α-螺旋,系统进化分为ε类和非ε类,ε类有12个14-3-3蛋白,含有4~5个蛋白编码区,非ε类有19个14-3-3蛋白,含有3~7个蛋白编码区。28个基因在所有组织中均有表达,但是表达丰度有差异,分别有5个基因在10 d的纤维中表达量较其它组织中高,表明14-3-3基因在纤维发育早期有重要作用。28个基因在高温、低温、盐和PEG处理表达量与对照相比有显著变化,并且这28个基因在高温或低温处理时表达量与对照相比均有差异,说明14-3-3蛋白对温度变化比较敏感。本研究解决了棉花14-3-3家族的基本信息,为棉花14-3-3基因家族的克隆和功能鉴定提供了指导意义。

In order to reveal the function of 14-3-3,we identified 31 14-3-3 in Gossypium hirsutum,and analyzed conservation structure domain,phylogenetic evolution,the physicochemical properties,gene structure,conservative motif and gene expression pattern in different tissue and various stress condition.The results showed that 31 14-3-3 proteins all contained nine classical reverse parallel helix.These genes were divided intoεand nonεsubfamilies.E subfamily contained 12 members,and had 4~5 protein coding regions.Nonεsubfamily contained19 members,and had 3~7 protein coding regions.Twenty-eight genes expressed in all tissues,but the expression abundance was different.Five genes expressed in 10 days fiber more than other tissues,indicating that 14-3-3 genes played an important role in the early stage of fiber development.Twenty-eight genes exhibited two-fold expression changes compared with controls in response to heat,salt,cold and polyethylene glycol(PEG).Moreover,all these 28 14-3-3 genes were differentially expressed under hot or cold stresses,indicating that 14-3-3 genes were sensitive to temperature.This results clarify the basic information of 14-3-3 family in cotton and provide basis information for further research on the function of 14-3-3 gene family members in Gossypium hirsutum.