利用重组自交系定位东乡野生稻在低氮胁迫下相关性状QTL

Identification of Related Traits QTL Stressed with Nitrogen Deficiency by Recombinant Inbred Lines of Dongxiang Wild Rice

东乡野生稻中有大量的优异基因,利用其片段替换系在低氮胁迫条件下进行相关性状的QTL定位,可为筛选耐低氮水稻材料提供有价值的参考。本研究以184个水稻重组自交系群体(东乡野生稻为供体亲本,籼稻保持系赣香B为受体亲本)为定位群体,利用完备区间作图法(ICIM)作图法,对正常与低氮胁迫条件下水稻有效穗、单株产量、株高、穗长和千粒重等性状进行QTL定位。共检测到100个QTLs,88个为新检测出的QTL,其中ph7b、nt1.1、nt3、nt6、pl9.3、tgw3a、tgw5a.1、tgw6a、tgw8a与前人研究结果相似,另外,pl7a.6和pl7b.2及pl8a.4和pl8b为在不同处理下均能影响穗长的位点;ypp5a和ypp5b为在不同处理下均能影响单株产量的位点;tgw2a.2和tgw2b、tgw3a和tgw3b、tgw5a.1和tgw5b.2及tgw8a和tgw8b为在不同处理条件下均能影响千粒重的位点。部分QTL与前人研究结果相似,新检测出来的QTL可能对筛选耐低氮水稻材料有所益处,也为水稻氮元素高效利用分子育种提供科学依据。

There are a large number of excellent genes in Dongxiang wild rice,which could provide valuable reference for the selection of low nitrogen tolerant rice materials by using its fragment replacement line to locate the related characters under low nitrogen stress conditions.This study took 184 rice recombinant inbred lines(Dongxiang wild rice as donor parent,indica rice Ganxiang B as the recipient parent)as the location population,and performed QTL location of the effective tiller,plant yield,plant height,panicle length and 1 000 grain weight of rice under normal and low nitrogen stress conditions by the inclusive composite interval mapping method.A total of100 QTLs were detected,of which 88 were newly detected QTLs,ph7 b,nt1.1,nt3,nt6,pl9.3,tgw3 a,tgw5 a.1,tgw6 a and tgw8 a are similar to those of previous studies.In addition,pl7 a.6,pl7 b.2,pl8 a.4 could affect the panicle length in different treatments.The locus of ypp5 a and ypp5 b affect the yield of single plant under different treatments;tgw2 a.2 and tgw2 b,tgw3 a and tgw3 b,tgw5 a.1 and tgw5 b.2,tgw8 a and tgw8 b affect 1 000 grain weight under different treatment conditions.Partial QTLs were similar to those of previous studies,the newly detected QTLs might be beneficial to the screening of low nitrogen tolerant rice materials,and also provide a scientific basis for molecular breeding of nitrogen efficient use in rice.