小麦穗粒数主效QTL-qKnps-4A遗传效应解析

Genetic Effects Analysis of Major QTL-qKnps-4A for Kernel per Spike in Common Wheat

结合前期穗粒数主效QTL-qKnps-4A定位结果及靶区段分子标记探针信息,通过3个巢式RIL群体4个环境穗粒数表型数据和188个‘科农9204’与‘京411’衍生的重组自交系群体(KJ-RIL)10个环境穗粒数表型数据进行了q Knps-4A物理定位分析,将其分别定位于Xwmc730-w Pt-8091(物理位置:4A:664209916-4A:736770981)与Ax-111729029-Ax-112286181(物理位置:4A:671926767-4A:684269262)物理区间。结合穗粒数主效稳定QTL紧密连锁标记(LOD峰值下标记)Ax-108966946基因型值,对188个KJ-RIL家系进行分组和产量性状表型数据方差分析,解析了来自京411的qKnps-4A优异等位基因对产量性状影响;结果表明qKnps-4A优异等位基因能够显著增加穗粒数及单株产量,对千粒重、单株穗数有一定的负向效应,平均单株产量增加6.06%。利用310份育成品种(系)在qKnps-4A靶区段基因型值,系统分析了靶区段优异单倍型的育种应用情况。结果表明,qKnps-4A优异单倍型已经被育种家选择,但还有较大的遗传改良空间。研究结果对穗粒数主效QTL-qKnps-4A遗传改良及分子育种应用提供理论参考。

Combined the orientation result of major QTL-qKnps-4 A of KPNS in earlier stage with molecular marker probe information in target segment,qKnps-4 A physical orientation analysis was carried out based on spike number phenotypic data of nested RIL colonies in 4 environments and spike number phenotypic data of recombinant inbred lines derived from 188'Kenong 9204'and'Jing 411'(KJ-RIL)in 10 environments,which were mapped to the physical interval between Xwmc730-wPt-8091(physical location:4 A:664209916-4 A:736770981)and Ax-111729029-Ax-112286181(physical location:4 A:671926767-4 A:684269262).Combined with the Ax-108966946 genotype value of major stable QTL tight linkage marker of KPNS(undermark of LOD peak),188 KJ-RIL families were grouped and anova was performed for the phenotypic data of yield traits,as well as the effect of qKnps-4 A superior allele from'Jing 411'on yield and traits were analyzed.The results showed that superior alleles of qKnps-4 A could significantly increase KNPS and kernel weight per plant(KWPP),but reduced thousand-kernel weight(TKW)and spike number per plant(SNPP)to some extent,and the average KWPP was increased by 6.06%.Based on the genotype value of 310 varieties/advanced lines in qKnps-4 A target segment,the breeding application of excellent haplotype in target segment was characterized.The results showed that the excellent haplotype of qKnps-4 A had been utilized by breeders,but there was great potential and space for genetic improvement.The present study would provide theoretical references for the genetic improvement and molecular breeding utilization of KNPS major qKnps-4 A.