绞股蓝皂苷XLIX对照品的制备与鉴定

Preparation and identification of gypenoside XLIX reference substance

目的研究绞股蓝皂苷XLIX的提取、分离、纯化和结构鉴定。方法采用正相制备色谱柱,以氯仿-甲醇梯度洗脱,得到绞股蓝皂苷XLIX粗品,再采用中压制备型液相色谱纯化,浓缩得绞股蓝皂苷XLIX纯品。UV、IR、MS、1H-NMR和13C-NMR测试数据确定结构。结果绞股蓝皂苷XLIX的分子式为C52H86O21,分子量为1046。结论测试数据结果与文献报导的gypenoside XLIX结构一致。

Objective To extract, isolate, purify and identify gypenoside XLIX. Methods Crude gypenoside XLIX was obtained by positive phase column with chloroform-methanol gradient elution, and medium pressure preparative liquid chromatography was used for purification. The pure gypenoside XLIX was concentrated. The structure was identified by UV-vis, IR, MS, 1 H-NMR and 13C-NMR. Results The molecular formula of gypenoside XLIX was C52H86O21 and its molecular weight was 1046. Conclusion The test results are consistent with gypenoside XLIX reported in the literature.