冠心病双生子差异表达mRNAs筛选及功能分析

Screening and functional analysis of differentially expressed mRNAs in twins with coronary heart disease

目的筛选冠心病-健康者双生子差异表达mRNAs,分析其在冠心病中的调控机制。方法选择3对冠心病-正常双生子为研究对象,其中3例冠心病患者为研究组,3例正常者为空白组。分别抽取2组受检者外周静脉血5 mL,Trizol法提取总RNA,Human Genome Array表达谱芯片筛选差异表达mRNAs,利用DAVID生物软件对差异表达mRNAs进行GO富集和KEGG通路分析。结果筛选出21个特异性差异表达mRNAs,其中3个上调,18个下调,这些基因主要参与了免疫调控。结论冠心病双生子存在差异表达的mRNAs,这些基因主要与免疫相关,参与了炎症因子的异常调控,可能是冠心病发生发展的关键病机之一。

Objective It is to screen differentially expressed mRNAs in coronary heart disease-healthy twins and to analyze their regulatory mechanisms in coronary heart disease.Methods Three pairs of coronary heart disease-normal twins were selected as the study subjects,in which the three patients with coronary heart disease were divided into study group,and the three healthy subjects were divided into blank group.5 mL peripheral venous blood was extracted from the subjects in the two groups,and total RNA was extracted by Trizol method.Human Genome Array expression microarray was used to screen differentially expressed mRNAs.The differentially expressed mRNAs were analyzed by DAVID bio-software for GO enrichment and KEGG pathway analysis.Results Twenty-one specific differentially expressed mRNAs were screened,of which 3 were up-regulated and 18 were down-regulated.These genes were mainly involved in immune regulation.Conclusion There are differentially expressed mRNAs in coronary heart disease twins.These genes are mainly related to immunity and participate in the abnormal regulation of inflammatory factors,which may be one of the key pathogenesis of coronary heart disease.