E-钙黏蛋白、β-链蛋白、MMP-16在食管癌组织中表达及其临床意义

Expression of E-cadherin,β-catenin and MMP-16 in esophageal carcinoma and its clinical significance

目的:探讨E-cadherin、β-catenin、MMP-16在食管癌组织中的表达情况及其临床意义。方法:收集74例食管癌患者的手术标本,采用免疫组化法检测E-cadherin、β-catenin、MMP-16的表达,记录患者临床病理参数并随访。结果:E-cadherin、β-catenin均主要表达于细胞膜,在癌旁组织中的阳性率显著高于食管癌组织。MMP-16主要表达于细胞膜,在食管癌组织中大量表达,阳性率显著高于癌旁组织。E-cadherin、β-catenin、MMP-16的表达与否与肿瘤分化程度、浸润程度、淋巴结转移、TNM分期相关(P<0.05)。E-cadherin与β-catenin表达呈正相关关系,与MMP-16呈负相关关系。β-catenin与MMP-16表达呈负相关关系。全组患者随访3~48个月,中位随访时间27个月。E-cadherin(+)组、E-cadherin(-)组的3年生存率分别为47.5%(28/59)、13.3%(2/15);β-catenin(+)组与β-catenin(-)组3年生存率分别为49.1%(28/57)、11.8%(2/17);MMP-16(+)组与MMP-16(-)组3年生存率分别为18.2%(10/55)、36.8%(7/19)。Log-rank检验发现,MMP-16(-)组、E-cadherin(+)组、β-catenin(+)组的预后均明显好于相应的对照组,差异均具有统计学意义(P<0.05)。结论:E-cadherin、β-catenin、MMP-16在食管癌患者中有一定的异常表达,且对患者的临床病理参数有一定的影响。

Objective:To investigate the expression and clinical significance of E-cadherin,β-catenin and MMP-16 in esophageal carcinoma.Methods:The surgical specimens of 74 patients with esophageal carcinoma were collected,and the expression of E-cadherin,β-catenin and MMP-16 was detected.Results:The positive rates of E-cadherin and β-catenin in adjacent tissues were significantly higher than those in esophageal carcinoma tissues.MMP-16 was mainly expressed in the cell membrane,and the positive rate was significantly higher than those in the adjacent tissues.The expression of E-cadherin,β-catenin and MMP-16 was correlated with tumor differentiation,infiltration,lymph node metastasis and TNM stage ( P <0.05).E-cadherin was positively correlated with the expression of β-catenin and negatively correlated with MMP-16.There was a negative correlation between the expression of MMP-16 and β-catenin.The 3 year survival rates of E-cadherin (+) group and E-cadherin(-) group were 47.5%(28/59) and 13.3%(2/15),and 49.1%(28/57) and 11.8%(2/17) in the group of β(+) andβ-catenin (-),and 18.2%(10/55) and 36.8%(7/19) in the MMP-16(+) group and MMP-16 (-) group,respectively.The results of Log-rank test showed that the prognosis of MMP-16 (-) group,E-cadherin (+) group and β-catenin (+) group was better than those of the corresponding control group,and the difference was statistically significant ( P <0.05).Conclusion:The abnormal expression of E-cadherin,β-catenin and MMP-16 have effects on the clinicopathological parameters and prognosis of patients.