氧浓度可控制小鼠饲养箱的研制

Development of a feeding box for mice with controllable oxygen concentration

目的:探索制作可以自动控制氧浓度的小鼠饲养箱的可行性,为制作早产儿视网膜病变模型提供帮助。方法:用粘合剂将有机玻璃粘合成透明密闭玻璃箱,前面设置开口便于放入和取出饲养小鼠的笼子,侧面设通风口,KY-2F型数字显示控氧仪置于玻璃箱顶部,其氧气探头和通过电磁阀的送气管从玻璃箱顶部接入箱内,连接控氧仪和电磁阀,用视网膜铺片和HE染色两种方法验证造模效果。结果:氧箱饲养的C57BL/6J新生小鼠第17d视网膜形成无灌注区并有新生血管生成,HE染色示视网膜新生血管突破内界膜。结论:本方法制作的可控制氧浓度的小鼠饲养箱简单易做、效果可靠。

AIM: To develop a new murine case with automatically controlled oxygen concentration for generating a mouse disease model of human retinopathy of prematurity (ROP). METHODS:An oxygen analyzer, an electromagnetic valve, and some of polymethyl methacrylate plates were purchased commercially and used for building up the device. A transparent sealed glass box was built with the organic glass and an adhesive. An opening was arranged in front of box for easy insertion and removal of the cage. A vent was arranged on the side. The KY-2F digital display oxygen controller and solenoid valve were placed on the top of the glass box. The oxygen probe and the air supply pipe through the solenoid valve were put into the box from the top of the glass box, Oxygen controller and solenoid valve were connected. Retina sheets and HE histologically staining were used to evaluate the animal model. RESULTS: Retina with non-perfusion area and neovascularization were observed in the C57BL/6J mice 17 th days after birth from the group in the case with controlled oxygen. Retina with HE staining showed that the neovascularization has penetrated over internal limiting membrane. CONCLUSION:The presented methodology here for generating mouse case with controlled oxygen is easy to do and apply, the model can truly mimic ROP histologically.