摘要
目的建立超高效液相色谱-串联质谱法测定猪肉中巴氯芬残留量的方法。方法样品采用0.1 moL/L盐酸-甲醇溶液(20:80,V:V)提取,混合型强阳离子交换柱(MCX)净化,经C18反相色谱柱分离后,正离子扫描,多反应监测模式检测巴氯芬,基质标曲定量。结果该方法在1~500 ng/mL浓度范围内线性良好,r2=1,检出限为1μg/kg,定量限为5μg/kg,回收率为75.28%~77.86%,精密度RSD<10%。结论该方法灵敏度高、特异性强,适用于猪肉中巴氯芬残留量的分析。
Objective To establish a method for the determination of baclofen in meat by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The sample was extracted by hydrochloride methanol, purified by solid-phase extraction cartridges (MCX) and separated on a reversed phase. Using ESI+ with multiple reactions monitoring (MRM), identification of baclofen residue was performed and quantified by matrix extracted calibration curve. Results Calibration curve was linear between 1 and 500 ng/mL with the correlation coefficient of 1. The limit of detection was 1 μg/kg and the limit of quantitation was 5 μg/kg. The recoveries were 75.28%-77.86%, and relative standard deviations (RSDs) were less than 10%. Conclusion This method has high sensitivity and high specificity, which is suitable for the determination of the baclofen residual amount in meat.
作者
严凤
潘娟
贡松松
胡申玥
张婧
吴剑平
黄士新
YAN Feng;PAN Juan;GONG Song-Song;HU Shen-Yue;ZHANG Jing;WU Jian-Ping;HUANG Shi-Xin(Shanghai Municipal Supervisory Institute Veterinary Drugs and Feedstaff, Shanghai 201103, China;Sanda University College of Food Quality and Safety Management, Shanghai 201209, China)
出处
《食品安全质量检测学报》
CAS
2019年第11期3390-3395,共6页
Journal of Food Safety and Quality
关键词
巴氯芬残留
猪肉
基质标准曲线
超高效液相色谱-串联质谱法
baclofen residue
meat
matrix extracted calibration
ultra performance liquid chromatographytandem mass spectrometry