饲用微生物添加剂地衣芽孢杆菌活菌计数不确定度评定方法的建立

Establishment of method for the uncertainty evaluation in viable count of Bacillus licheniformis as feed microorganism additive

目的探讨检测实验室饲用微生物添加剂地衣芽孢杆菌活菌计数的不确定度评价方法。方法实验依据JJF1059.1-2012《测量不确定度评定与表示》、RB/T151-2016《食品微生物定量检测的测量不确定度评估指南》和NY/T1461-2007《饲料微生物添加剂地衣芽孢杆菌》,对地衣芽孢杆菌活菌计数的不确定度分量进行分析。结果本研究建立了地衣芽孢杆菌活菌计数不确定度评定方法,当包含因子k=2时,扩展不确定度U=4.05%,样品的误差允许范围在0.95×10^10~1.15×10^10CFU/g之间。结论建立的地衣芽孢杆菌活菌计数不确定度评定方法简单、易行,符合实际检测要求。

Objective To explore the method of uncertainty evaluation for viable count of Bacillus Licheniformis as feed microorganism additive. Methods The uncertainty components were analyzed according to JJF 1059.1-2012 Evaluation and representation of measurement uncertainty, RB/T 151-2016 Guidelines for the estimation of measurement uncertainty of food microbiological quantitative detection and NY/T 1461-2007 Bacillus licheniformis as feed microbial additives. Results This method could evaluate the uncertainty of viable count of Bacillus licheniformis. When the factor k=2, the expanded uncertainty U was 4.05%. The allowable error range of the sample were between 0.95×10^10-1.15×10^10 CFU/g. Conclusion This method for evaluating the uncertainty of viable count of Bacillus licheniformis is simple and feasible, which meets the actual detection requirements.