蛋白与低聚半乳糖复合粉增强免疫功能的实验研究

Experimental study on the enhancement of immune function by complex powder of protein and galactoligosaccharide

目的评价蛋白粉与低聚半乳糖复合粉对小鼠免疫功能的影响。方法按《保健食品检验与评价技术规范》2003版中增强免疫力功能检验方法,采取灌胃方式,按照小鼠0.83、1.67、5.0 g/(kg?bw)剂量给予蛋白复合粉30 d后,研究对小鼠细胞免疫、体液免疫、单核-巨噬细胞功能、NK细胞活性功能的影响。结果ConA诱导的小鼠脾淋巴细胞转化实验及迟发型变态反应实验中,高剂量组(5.0 g/(kg?bw))与对照组比较,数据有显著差异(P<0.05)。碳廓清实验中,中剂量组(1.67 g/(kg?bw))、高剂量组(5.0 g/(kg?bw))的吞噬指数a比对照组明显增加(P<0.05)。NK细胞活性在中剂量组(1.67 g/(kg?bw))、高剂量组(5.0 g/(kg?bw))与对照组间比较有显著性差异(P<0.05)。各剂量组对小鼠体重增长、胸腺指数、脾指数,血清溶血素水平和抗体生成细胞数均无显著性影响。结论蛋白复合粉能够增强细胞免疫功能、单核巨噬细胞功能,提高NK细胞活性,说明蛋白复合粉具有增强免疫力的作用。

Objective To evaluate the effect of a complex powder of protein and galactooligosaccharide (GOS) on the mouse immune function. Methods In accordance with methods for the assessment of enhancing immune function in Technical standards for testing and assessment of health food, intragastric administration was carried out, and mice were given by gavage with the protein complex powder at 0.83, 1.67, 5.0 g/(kg?bw) doses for 30 d. The effects on cellular immunity, humoral immunity, single nucleus-macrophage function and NK cell activity in mice were studied. Results The lymphocyte proliferation ability of mice induced by ConA and delayed-type hypersensitivity response in high-dose group (5.0 g/(kg?bw)) were obviously higher than control group. Compared with the control group, the carbon clearance capacity test in the medium-dose and high-dose groups of 1.67 g/( kg?bw) and 5.0 g/( kg?bw) could remarkably enhance the phagocytic index a (P<0.05). The NK cell activities of mice in the medium-dose and high-dose groups of 1.67 and 5.0 g/(kg?bw) were significantly higher than that of the control group. There were no significant differences between the control group and the experiment groups in the matters of body weight gain, thymus index, spleen index, serum hemolysin level and antibody level. Conclusion Protein complex powder can enhance the function of cellular immunity, mononuclear macrophages and NK cells, indicating that it could enhance immunity function.