摘要
基于表面增强拉曼光谱技术(SERS),采用自制表面增强试剂(纳米金)与厂家提供的表面增强试剂,对比研究了对21种查缴的保健品样品中的非法添加物西地那非的快速拉曼检测效果,同时考察了不同配比纳米金和不同浓度的盐酸、硝酸和硫酸作为助剂的增强效果。结果表明,自制纳米金表面增强试剂(氯金酸和柠檬酸钠的体积比1∶1)及2.67 mol·L^-1的硝酸表面增强效果为佳,可对浓度为0.01μg·mL^-1西地那非阳性检出;与飞行时间-液质联用仪(QTOF-LC-MS)分析结果对比,自制纳米金检测吻合度为100%,厂家提供的增强试剂检测吻合度为86.4%。综上,自制表面增强试剂(纳米金)表面增强效果不差于厂家提供的表面增强试剂。
Based on surface-enhanced raman spectroscopy(SERS),the illegal additive sildenafil in 21 samples of seized health care products was detected by self-made nano-gold as the surface-enhanced reagents comparing with those provided by the manufacturers. The enhancement effects of nano-gold in various proportions with using diverse concentrations of hydrochloric acid,nitric acid and sulfuric acid were additionally measured. The results showed that:As the minimum detection limit of sildenafil was 0.01 μg·mL^-1,the enhancement effect of self-made nano-gold as the surface-enhanced reagent(the volume of cholor-gold acid and sodium citrate was in the proportion 1∶1)added nitric acid with the concentration of 2.67 mol·L^-1 was as result the best among the all tests,so that it was 100% consistent with the results analyzed by QTOF-LC-MS,while those provided by the manufacturer were only 86.4% consistent. This indicated that the surface enhancement effect of the self-made surface enhancement reagent(nano-gold)was not inferior to that provided by the manufacturer.
作者
吴国萍
周亚红
李静泉
李家伟
WU Guo-ping;ZHOU Ya-hong;LI Jing-quan;LI Jia-wei(Department of Criminal Science and Technology,Jiangsu Police Institute,Nanjing 210031,China;Jiangsu Engineering Laboratory of Food,Drug,and Environment Criminal Investigation and Forensic Analysis,Nanjing 210031,China)
出处
《食品工业科技》
CAS
北大核心
2019年第11期254-259,264,共7页
Science and Technology of Food Industry
基金
公安部软课题项目(2016LLYJJSST025)
江苏省教育厅自然科学重大项目(18KJA620001)
江苏警官学院科研创新团队(2018SJYTD03)
“十三五”江苏省重点建设学科建设工程
关键词
表面增强拉曼光谱
保健品纳米金
西地那非
surface enhanced Raman spectroscopy
health care products
nano-gold
sildenafil