糖尿病斑马鱼模型的建立与南极磷虾酶解物降血糖活性评价

Establishment of diabetic zebrafish model and evaluation of hypoglycemic activity of antarctic krill enzymatic hydrolysates

采用联合20g/L葡萄糖溶液浸泡和10%胆固醇喂养20d快速建立了稳定的糖尿病斑马鱼模型,并用该模型对3 000~100Da南极磷虾酶解物(AKEH)的体内降血糖效果进行评价,具体考察AKEH对糖尿病斑马鱼DPP-IV的活性、理化指标(葡萄糖、甘油三酯、胆固醇)和与血糖水平相关基因(Insa、Glucagon、Pck1)表达水平的影响。结果表明:与阴性对照组相比,低剂量的3 000~100Da AKEH组分(1.35,2.70g/L)对糖尿病斑马鱼的理化指标与血糖相关基因表达水平影响不显著,而高剂量的3 000~100Da AKEH(5.40g/L)能降低其血糖的理化指标水平,显著抑制糖尿病斑马鱼DPP-IV活性与Glucagon基因和Pck1基因的表达水平、提高Insa基因表达水平。因此,3 000~100Da AKEH组分具有一定的降血糖功效。

Diabetic zebrafish model was established quickly by combination of 20 g/L glucose solution immersing and 10% cholesterol feeding for 20 days. The model established was used to evaluate the in vivo hypoglycemic activity of 3 000~100 Da AKEH. The effects of it on physicochemical indexes such as DPP-IV activity, glucose, triglyceride, cholesterol and expression levels of relative gene such as Insa , Glucagon and Pck1 of diabetic zebrafish were investigated. The results showed that the physicochemical index and blood glucose related gene expression level of the 1.35 g/L and 2.7 g/L concentrations were not significant difference. 3 000~100 Da AKEH of 5.4 g/L could be reduced the level of physicochemical index, significantly inhibited DPP-IV activity, the expression level of Glucagon and Pck1 gene and improved Insa expression level. Therefore, 3 000~100 Da AKEH has certain hypoglycemic effects.