基于~1H-NMR的原发性肝癌脾虚湿盛证患者尿液代谢组学研究

Urine Metabonomics Studies on Primary Liver Cancer Patients of Damp Abundance Due to Spleen Asthenia Syndrome with Proton Nuclear Magnetic Resonance Spectroscopy

目的 通过对原发性肝癌脾虚湿盛证患者尿液代谢组学研究,以探讨原发性肝癌脾虚湿盛证患者代谢变化机理。方法 选取35例脾虚湿盛证肝癌患者、35例非脾虚湿盛证肝癌患者和40例和平质健康体检者的尿液进行核磁共振氢谱检测(proton nuclear magnetic resonance spectroscopy,1H-NMR),谱图积分结果使用正交偏最小二乘判别分析法(partial least square discriminant analysis,PLS-DA)进行分析。结果 原发性肝癌患者尿中肌酐、马尿酸、氧化三甲胺(TMAO)、柠檬酸、丙氨酸、亮氨酸、谷氨酸含量均较较对照组健康人低(P<0.01),且脾虚湿盛证组低于非脾虚湿盛证组(P<0.05);而牛磺酸、琥珀酸的含量显著高于对照组,且脾虚湿盛证组高于非脾虚湿盛证组(P<0.05),差异具有统计学意义。原发性肝癌患者尿中乙酸含量显著性高于对照组(P<0.05),而在脾虚湿盛证组与非脾虚湿盛证组中比较,无统计学差异。结论 原发性肝癌患者存在糖代谢、脂类代谢、氨基酸分解代谢紊乱,且原发性肝癌脾虚湿盛证患者体内多种生命代谢的紊乱程度较非脾虚湿盛证患者严重。

Objective To observe metabolomic changes in urine of Primary Liver Cancer Patients of Damp Abundance due to Spleen Asthenia Syndrome with proton nuclear magnetic resonance spectroscopy(1H-NMR). Methods 1H-NMR based metabonomics analysis was performed on urine samples from 35 Liver Cancer patients with Damp Abundance due to Spleen Asthenia Syndrome,35 Liver Cancer patients without the syndrome,and 40 Peaceful and healthy ubjects. Results The level of Creatinine,equuric acid,TMAO,citric acid,alanine,leucine,glutamate in Primary Liver Cancer Patients was lower than that in healthy subjects and they in Liver Cancer patients with Damp Abundance due to Spleen Asthenia Syndrome was lower than that in patients without the syndrome.The level of Taurine,succinic acid in Liver Cancer patients with Damp Abundance due to Spleen Asthenia Syndrome was higher than that in patients without the syndrome.There was statistical significance( P <0.05).The level of acetic acid in Primary Liver Cancer Patients was significantly higher than in healthy subjects. Conclusion Primary Liver Cancer Patients have disorder about glycometabolism,lipid metabolism,and amino acids catabolism and in Primary Liver Cancer Patients of Damp Abundance due to Spleen Asthenia Syndrome are much more serious than that of Liver Cancer patients without the syndrome.