摘要
目的:对16个不同居群的中麻黄从23对叶绿体编码区及非编码区DNA片段中筛选具有变异的叶绿体DNA片段。方法:通过试剂盒法提取DNA并梯度优化筛选扩增条件,PCR扩增产物经生物工程有限公司测序获得产物序列。结果:引物[trnS(GCU)-trnG(UCC)、atpB-rbcL、psbA-trnH、F71-R1516]能扩增出清晰、单一的条带。结论:16个不同居群中30个麻黄个体的叶绿体DNA扩增成功,初步筛选出具有变异的叶绿体DNA片段F71-R1516。
The Chloroplast DNA fragments with mutations from 23 pairs of chloroplast coding regions and non-coding regions were screened in 16 different populations of Ephedra intermedia. Methods:DNA was extracted and the amplification conditions were selected by gradient optimization. The PCR amplification products were sequenced by Bioengineering Co.,Ltd. to obtain the sequence.Results:The clear,single bands were obtained by primers(trnS(GCU)-trnG( UCC),atpB-rbcL,psbA-trnH,F71-R1516). Conclusion:Thirty individuals from 16 ephedra populations of chloroplast DNA were successfully amplified,and the chloroplast DNA fragments F71-R1516 with mutation were screened.
作者
马晓辉
晋玲
朱田田
MA Xiaohui;JIN Ling;ZHU Tiantian(Gansu University of Chinese Medicine, Lanzhou Gansu 730000, China;Research Institute of Chinese (Tibetan) Medicinal Resources,Lanzhou Gansu 730000, China;Key Laboratory of Chemistry and Quality for TCM and Tibetan Medicine in Universities of Gansu Province, Lanzhou Gansu 730000, China)
出处
《中兽医医药杂志》
2019年第3期11-13,共3页
Journal of Traditional Chinese Veterinary Medicine
基金
2014高校基本科研业务费专项课题(甘财教[2014]63号06课题)
科技基础性工作专项重点项目子课题(SB2007FY020)
中央财政引导地方科技创新平台项目资助"甘肃省中药资源与新产品开发平台建设"
甘肃省科技计划基础研究创新群体资助(1606RJIA323)
关键词
中麻黄
叶绿体
引物
条件筛选
Ephedra intermedia Schrenk et C. A. Mey
chloroplast
primer
conditional screening
