一种基于分子内电荷转移的亚硫酸氢盐比率测量荧光探针

A Ratiometric Fluorescent Probe for Bisulfite Based on Intramolecular Charge Transfer

2-甲基苯并噻唑与对苯二甲醛一步反应制得含有苯甲醛结构的半菁染料HCy,通过1H NMR与MS对目标染料HCy进行了表征。考察了HCy在水溶液及DMSO溶液中对HSO3-的识别性能以及不同环境对HCy光谱的影响,并测试了HCy对肝细胞(HL-7702)与肝癌细胞(Hep G2)的毒性。结果显示,荧光探针HCy对亚硫酸氢盐显示出优异的选择性及高灵敏度,在水溶液中及DMSO溶液中均可快速响应,适宜于作为HSO3-的比率测量荧光探针;此外,HCy对GSH有特异性响应,细胞实验表明,HCy具有优异的生物相容性。

In this paper,A hemicyanine dye(HCy)which contains benzaldehyde structure was prepared by one-step reaction of 2-methylbenzothiazole with terephthalaldehyde,Its structure was characterized by 1H NMR and MS.The recognition of HCy to HSO3^-in both aqueous and DMSO solution,and the effects on the spectral properties of HCy caused by different environments were tested.The toxicity of HCy to hepatocytes(HL-7702)and hepatoma cells(HepG2)were also investigated.The results showed that this benzaldehyde-based fluorescent probe exhibits excellent selectivity and sensitivity to bisulfite in both aqueous and DMSO solution.In addition,our results also showed that HCy can respond to GSH specifically,and HCy has excellent biocompatibility in the cell experiments.