颈动脉低灌注大鼠模型海马区IL-33和cleaved-caspase-3的表达

Expression of IL-33 and cleaved-caspase-3 in the hippocampus of rats with carotid artery hypoperfusion

目的探讨IL-33在颈动脉低灌注导致的认知障碍中的可能作用。方法36只成年雄性SD大鼠随机分为3组:假手术(S)组,模型2周(L2w)组,模型4周(L4w)组。Morris水迷宫测试各组大鼠学习记忆能力;免疫组化法检测海马区deaved-caspase-3表达水平;Western-blot法检测海马区IL-33表达水平。结果水迷宫测试、cleaved-caspase-3表达和IL-33表达,3组之间比较差异均有统计学意义(F=64.201,P<0.05;F=233.558,P<0.05;F=51.498,P<0.05)。与S组(20.32±6.30)s比较,L2w组(46.67±9.49)s逃逸潜伏期显著延长(t=-4.902,P<0.05);L4w组(81.51±14.67)s与L2W组比较,逃逸潜伏期显著延长(t=-6.397,P<0.05)。与S组(1.31±1.19)比较,L2w组(6.56±1.32)海马区deaved-caspase-3表达显著增加(t=-6.328,P<0.05);与L2w组比较,L4w组(18.78±5.83)海马区cleaved-caspase-3表达显著增加(t=-14.733,P<0.05)。与S组(0.26±0.02)比较,L2w组(0.3±0.04)海马区IL-33表达有增加趋势,但结果无统计学意义(t=-1.530,P=0.147);与L2w组比较,L4w组(0.49±0.06)海马区IL-33表达显著增加(t=-7.924,P<0.05)。结论IL-33可能在脑组织低灌注导致的认知障碍中起作用。

Objectives To in v estig ate the possible role of interleukin -33 (IL -33 ) in cognitive impairment caused by hypoperfusion of the carotid artery. Methods A total of 36 adult male Sprague-Dawley (SD) rats were random ly divided into 3 groups : sham operation (S) group , Lesion 2 weeks (L2w) group , and Lesion 4 weeks (L4w) group. The Morris water maze was used to test the learning and memory ability of each group. The expression of cleaved-caspase-3 in the hippocam pus was tested by im m unohistochem istry. The expression of IL-33 in the hippocam pus was tested by Western-blot method. Results The differences in the water maze test perform ance and the expression of cleaved-caspase-3 and IL-33 were statistically significant between the three groups (F = 64.201 , P<0.05;F=233.558, P<0.05;F = 51.498, P<0.05). Compared with the S group (20.32 ± 6.30 )s, the escape latency of the L2w group (46.67 ± 9.49) s was significantly prolonged G=-4.902 , P<0.05);com pared with the L2w group, the escape latency of the L4w group (81.51 ± 14.67)s was significantly prolonged (t=-6.397 , P < 0.05). Compared with the S group (1.31 ± 1.19), the expression of cleaved-caspase-3 in the hippocam pus in the L2w group (6.56 ± 1.32) was significantly increased t =-6.3 28, P<0.05);compared with the L2w group, the L4w group’s (18.78 ± 5.83) expression of cleaved-caspase-3 in the hippocam pus was significantly increased (t=-14.733, P < 0.05). Compared with the S group (0.26 ± 0.02), the expression of IL-33 in hippocam pus in the L2w group (0.3 ± 0.04) in creased , but the results were not statistically significant(t=-1 .530 , P = 0.147 );com pared with the L2w group, the L4w group s expression of IL-33 in the hippocam pus (0.49 ± 0.06) was significantly increased (t=-7.924 , P <0.05). Conclusions IL-33 may play a role in cognitive impairment caused by hypoperfusion of brain tissue.