滩羊微卫星标记多态性及与体尺性状关联分析

Microsatellite Polymorphism and Its Correlation Analysis with Body Size Traits of Tan Sheep

滩羊是我国特有的蒙古羊品种,肉质鲜美但生长缓慢。通过筛选的29 个高多态性微卫星标记,对宁夏盐池滩羊种羊场基础母羊群体用随机抽样法检测了96 个成年个体,测量了体重和体尺数据,分析了其微卫星标记遗传多样性、群体遗传结构及分子标记与表型的相关关系。结果显示,微卫星标记平均等位基因数9.5,平均有效等位基因数4.5,平均期望杂合度0.72,平均观测杂合度0.64,平均多态信息含量0.69;滩羊基础母羊群体保持了丰富的遗传多样性;关联分析结果显示MAF33 与滩羊的体高、胸深显著相关,BMS1788 与荐高、胸围显著相关,而BL41、BMS835、BOVILS56、MAF33 和BMS500 与管围极显著关联。这些标记对未来滩羊的分子辅助育种有所帮助。

Tan sheep,a special Mongolia sheep breed in China,is famous with its delicious meat and slow growth rate. In our study, a total of 29 high polymorphic microsatellite markers were used to screen 96 Tan sheep individuals,which were sampled from the basic ewe population in Yanchi Tan sheep reservation farm in the Ningxia Hui Autonomous Region,China. Analysis of genetic diversity,population structure and co-relationship between molecular genotype and measured phenotype(body weight and body size traits)of the 96 samples were carried out,and the following results were obtained :the average(Na)and mean effective(Ne)numbers of alleles,the average expected heterozygosity(He),the average observed heterozygosity(Ho),and the average polymorphism information content(PIC)were 9.5,4.5, 0.72,0.64 and 0.69,respectively. The sampled population held high genetic diversity,the correlation between MAF33 and body height and chest depth existed,and BMS1788 was associated with rump height and chest circumference. Also,5 microsatellite markers including BL41, BMS835,BOVILS56,MAF33 and BMS500 were significantly correlated with the cannon circumference. These six microsatellite loci may be useful in further marker-assisted breeding for Tan sheep.