基于HPLC-ELSD和偏最小二乘判别分析法的蜂蜜掺假鉴别

Identification of Adulterated Honey Based on HPLC-ELSD and Partial Least Squares-Discriminant Analysis

为了实现蜂蜜掺假的简便、快速识别,应用高效液相色谱技术和化学计量学手段建立了一种蜂蜜掺假检测的新方法。该研究收集了中国不同品种、不同地域的典型天然蜂蜜样品,根据目前市场上常见的蜂蜜掺假手段、掺假物质及相对含量情况模拟制备系列掺假蜂蜜样品,利用HPLC-ELSD对样品中糖类物质进行分离测定,并采用PLS-DA统计学方法进行蜂蜜掺假识别研究。研究结果表明:掺入质量分数10%以上果葡糖浆和麦芽糖浆的掺假蜂蜜样品均能利用本方法予以鉴别,其校正集的正确判别率达到92.5%,验证集的正确判别率为100%。

In order to identify the honey adulteration,this paper present a new high performance liquid chromatography method,coupled with chemometric analysis,used to identification of adulterated honey.The sugar composibion of 33 honey samples obtained from different areas were separated and detected by HPLC-ELSD equipped with Amide column,The method relies on the use of chemometric methods to process chromatograms in order to achieve a better discrimination between authentic and adulterated honeys by partial least squares-discriminant analysis(PLS-DA).This approach was investigated using honey samples adulterated from 10%to 30%with high fructose syrup and maltose syrup.Good result were obtained in the characterization of authentic and adulterated samples:92.5%of good classification in the training set and 100%in the test set.