苦水玫瑰干花蕾中总黄酮的提取工艺及其主要成分的测定

Extraction Total Flavonoids and Determination of Main Compounds in Kushui Rose

采用响应面分析法优化苦水玫瑰中总黄酮的提取工艺,在此基础上使用超高效合相色谱(Ultra Performance Convergence Chromatography,UPC^2)对总黄酮中的槲皮素和山奈酚的质量分数进行检测。以提取溶剂、液料体积质量比、提取时间和提取温度为响应因子,总黄酮得率为响应值,采用3因素3水平的响应面分析法优化苦水玫瑰中总黄酮的提取工艺参数。结果表明,最佳提取工艺条件为乙醇体积分数66%、液料体积质量比16 mL/g、提取时间120 min,提取温度70℃,在此最优条件下,分别做3次平行实验进行验证,总黄酮提取量为5.85 mg/g,与模型预测值5.87 mg/g基本相符。说明了响应面模型可较好地预测苦水玫瑰总黄酮的提取量,对提取条件参数的优化具有可行性。实验还研究了苦水玫瑰总黄酮中槲皮素和山奈酚的测定方法。槲皮素和山奈酚在一定范围内线性良好,相关系数为0.998 2~0.999 3;精密度RSD均低于1.76%;平均回收率为96.05%~101.15%。该方法能够满足苦水玫瑰总黄酮中槲皮素和山奈酚的快速检测。

Surface response optimized and extraction total flavonoids and determination of main compounds in kushui rose,under this condition,using Ultra Performance Convergence Chromatography(UPC^2)to determined quercetin and kaempferol in total flavonoids.The parameters of extraction technology of total flavonoids from kushui rose were optimized by four factors and three levels response methodology using ethanol concentration,issue-liquid ratio,extraction time and extraction temperature as the response factors and with yeild rate of total flavonoids as response value.The results shows that the optimal extraction technology was as follows:ethanol concentration66%,issue-liquid ratio 16:1(mL/g),extraction time 120 min and extraction temperature 70℃.Under these conditions,every sample was tested three times,the maximal yield of total flavonoids reached 5.85 mg/g.The total flavonoids the same as model predicated contents which was 5.87 mg/g.The technology can be used as the extraction process of total flavonoids from kushui rose.This article also studied the content of quercetin and kaempferol in total flavonoids.Quercetin and kaempferol in a range of good linearity,the correlation coefficient was 0.998 2~0.999 3;precision(RSD)were less than 1.76%;the average recovery was 96.05%~101.15%.This methods was suitable to rapid detection of quercetin and kaempferol in total flavonoids.