枝睾阔盘吸虫凉山州分离株nad4基因部分序列测定与种系发育分析

Sequence and Phylogenetic Analysis of nad4 Gene of Eurytrema cladorchis Isolated from Liangshan

为探究山羊源枝睾阔盘吸虫的种内遗传变异和系统发育关系,对采集自四川省凉山州的5个吸虫样品nad4基因部分序列进行PCR扩增、序列测定及分析,并构建种系发育树。测序结果显示,所测得的5个山羊源枝睾阔盘吸虫nad4基因部分序列长度均为789bp,核苷酸同源性为99.5%~100.0%,共检测到4个单倍型、4个变异位点。系统发育树显示,5个山羊源枝睾阔盘吸虫凉山州分离株聚在同一小支上,未与胰阔盘吸虫聚类在一支上,能与其他吸虫相鉴别。本研究结果为枝睾阔盘吸虫的分子分类和种群遗传的进一步研究奠定了基础。

In order to study the genetic variations of Eurytrema cladorchis and the phylogentic relationships with other flukes,fragment of nad4 gene of 5 flukes isolated from Liangshan Prefecture in Sichuan Province were amplified by PCR,then analyzed the sequences,and the NJ tree was reconstructed.The results showed that the partial sequences of nad4 gene were 789 bp,with homology was 99.5%-100.0%,four haplotypes were detected in these sequences, with 4 variable sites.The phylogenetic tree showed that the isolates of E. cladorchis from goats in Liangshan were clustered in the same clade, which were not cluster together with E. pancreaticum, can be identified with other flukes.The results of this study could provide the foundation for further research on the molecular classification and population genetics of E. cladorchis.