莫西沙星增加哮喘大鼠气道平滑肌细胞Caveolin-1的表达

Moxifloxacin could increase the expression of caveolin-1 of airway smooth muscle cell of asthmatic rat

目的观察在莫西沙星作用下,支气管哮喘(哮喘)大鼠气道平滑肌细胞(ASMC)小凹蛋白-1(Cav-1)蛋白及mRNA表达的变化,探讨莫西沙星对ASMC发挥抗炎调节的可能机制。方法用随机数字表法将40只SD大鼠随机分为正常组(10只)及哮喘组(30只)。复制哮喘大鼠模型,体外传代培养正常及哮喘组大鼠ASMC,正常组作为空白对照组(A组)、哮喘组分为哮喘对照组(B组)、莫西沙星干预组(C组,莫西沙星5mg/L干预哮喘模型细胞)、地塞米松干预组(D组,地塞米松10-6mol/L干预哮喘模型细胞),均孵育48h后,分别应用Westernblot和qRT-PCR检测Cav-1蛋白水平及mRNA表达。结果Westernblot检测显示,B组Cav-1蛋白表达量(1.16±0.05)较A、C、D组[Cav-1蛋白表达量(3.56±0.05,2.86±0.11,3.01±0.17)]降低,差异有统计学意义(P值均<0.05)。C组与D组之间Cav-1蛋白表达量差异无统计学意义(P=0.172)。qRT-PCR结果显示B组Cav-1mRNA表达量(1.00±0.11)较A、C、D组[mRNA表达量分别为(3.26±0.22,2.68±0.11,2.93±0.05)]降低,差异有统计学意义(P值均<0.05),C组与D组之间Cav-1mRNA表达量差异无统计学意义(P=0.052)。结论莫西沙星可增加哮喘大鼠ASMCCav-1蛋白及mRNA表达,这可能与莫西沙星对ASMC发挥抗炎作用相关。

Objective To observe the effects of moxifloxacin on the expression of the protein and mRNA of caveolin-1 (Cav-1) in asthmatic rat, and to explore the possible mechanisms of anti-inflammatory effects of moxifloxacin on airway smooth muscle cell (ASMC). Methods Totally 40 male SD rats were randomly and equally divided into normal group (10) and asthmatic group (30). Asthma model were established.ASMC were derived from normal and asthmatic rats and cultured in vitro.The normal group was regarded as normal control group (A), the asthmatic group was divided into 3 groups, including asthmatic control group (B), asthmatic group (C) to which moxifloxacin was added at the concentration of 5 mg/L and asthmatic group (D) to which Dexamethasone was added at the concentration of 10-6 mol/L.Then the cells of different groups were incubated for 48 h. Finally the protein of Cav-1 was detected by Western blot and the mRNA of Cav-1 was measured by qRT-PCT respectively. Results The results of Western blot indicated that the expression of Cav-1 protein of group B (1.16±0.05) decreased compared with groups A, C, D (the expression of Cav-1 protein of these groups was 3.56±0.05, 2.86±0.11, 3.01±0.17 respectively)(P<0.05). But there were no significantly difference between groups C and D (P=0.172). The results of qRT-PCR showed that the expression of mRNA of group B (1.00±0.11) decreased compared with groups A, C, D (the expression of mRNA of these groups were 3.26±0.22, 2.68±0.11, 2.93±0.05 respectively)(P<0.05). But there were no significantly difference between groups C and D (P=0.052). Conclusions Moxifloxacin can increase the protein and mRNA of Cav-1 in asthmatic rat ASMC, which may be the possible mechanism concerning the anti-inflammatory effects of moxifloxacin.