Box-Behnken法优化大叶榉树黄酮类超声辅助提取方法及初步鉴定

Optimization of Ultrasonic Extraction of Flavonoids from Zelkova schneideriana Leaves by Box-Behnken

为了探讨大叶榉树Zelkova schneideriana叶片黄酮类成分,以1年生大叶榉树叶片为材料,在超声波辅助下,通过单因素和Box-Behnken试验,探究并优化了大叶榉叶片黄酮类乙醇提取方法;采用常规显色法,对大叶榉树叶片中黄酮类物质种类进行初步鉴定。结果表明:(1)优化后的大叶榉树黄酮类提取方法为:乙醇浓度63%、液料比31:1mL·g^-1、提取时间1.7 h,大叶榉树黄酮类提取量为(31.067±0.11)mg·g^-1;(2)初步鉴定发现:大叶榉树叶片中含有黄酮、具有邻二酚羟基结构的黄酮类化合物、具有邻二苯酚羟基或兼有3-OH,4=O或5-OH,4=O的化合物;可能有黄酮醇、二氢黄酮、二氢黄酮醇等;不含查尔酮和橙酮。

On August 26, 2017, collections were implemented on1-3 leaves of 1-year Zelkova schneideriana in greenhouse for experiment on extraction of flavonoids. Optimization of ultrasonic extraction of flavonoids was designed by box-behnken with three factors at three levels. The result demonstrated that the best technology for extraction was as follows, ethanol concentration of 63%, liquid ratio of 31:1 mL·g^-1, extraction time of 1.7 hour, with the yield of flavonoids of(31.067±0.11)mL·g^-1. Preliminary identification of extracted flavonoids showed flavones, flavonoids with adjacent two phenolic hydroxyl structures, and compounds with two phenol hydroxyl groups or 3-OH, 4=O or 5-OH, 4=O, and possible flavonoils, dihydroflavones and dihydro flavonols, but no chalcone and aurones.