醋糟固态发酵产植酸酶的工艺研究

Study about Phytase Production from Vinegar Grains by Solid State Fermentation

以醋糟为固体培养基的主要成分,以植酸酶活力为指标,通过单因素和正交试验,优化里氏木霉固体发酵产植酸酶的培养基组成及培养条件。即在蔗糖添加量为4%,蛋白胨添加量为1.5%,吐温-80添加量为0.4%,摇床转速220r·min^-1,温度为30℃,接种量为4%时,酶活力为2.9U·mL^-1。

Vinegar grains serve as the main component of solid medium and phytase activity serve as our standard measured, the composition and cultured conditions that Trichoderma reesei produce phytase were optimized via single factor and orthogonal experiments. The detailed conditions are sucrose added in an amount of 4%, peptone in an amount of 1.5%, tween-80 in an amount of 0.4%, inoculation in an amount of 4%,shaking in the speed of 220 r·min^-1 at temperature 30 ℃ and the enzyme activity is 2.9 U·mL^-1.