ANXA1对口腔鳞癌TPF化疗敏感性的影响及作用机制探讨

The effect and mechanism of ANXA1 on TPF chemosensitivity in oral squamous cell carcinoma

目的:探讨ANXA1在口腔鳞癌TPF化疗中的作用机制。方法:构建ANXA1过表达及低表达细胞系,通过细胞增殖、细胞毒性实验、实时荧光定量PCR和Western免疫印迹方法,分析ANXA1在TPF化疗中的作用,并探讨ANXA1通过上皮-间质转化(EMT)通路在TPF化疗中的作用机制。采用SPSS18.0软件包对数据进行统计学分析。结果:过表达口腔鳞癌细胞系ANXA1后,细胞生长速率降低,细胞周期减慢,对TPF诱导化药物敏感性降低,口腔鳞癌细胞出现EMT;低表达口腔鳞癌细胞系ANXA1后,细胞生长速率增快,细胞周期加快,对TPF化疗药物敏感性增加,口腔鳞癌细胞出现逆EMT。结论:在口腔鳞癌TPF化疗中,ANXA1过表达导致细胞出现EMT,对化疗敏感性降低。

PURPOSE:To investigate the mechanism of ANXA1 in TPF chemotherapy of oral squamous cell carcinoma(OSCC).METHODS:ANXA1 overexpression and low-expression cell lines were constructed.The role of ANXA1 in TPF chemotherapy was analyzed by cell proliferation,cytotoxicity test,real-time PCR and Western blot,and the mechanism of ANXA1 in TPF chemotherapy through EMT(epithelial-mesenchymal transition) pathway was discussed.The data were analyzed with SPSS 18.0 software package.RESULTS:After overexpression of ANXA1,cell growth rate decreased,cell cycle slowed down,sensitivity to TPF-induced drugs decreased,and EMT occurred in OSCC.After underexpression of ANXA1,cell growth rate increased,cell cycle accelerated,sensitivity to TPF chemotherapeutic drugs increased,and reverse EMT occurred in OSCC.CONCLUSIONS:In TPF chemotherapy of OSCC,overexpression of ANXA1 results in EMT of cells,which leads to decreased chemosensitivity.