CTLA-4在肺腺癌细胞及其肿瘤浸润性淋巴细胞中表达的意义

Significance of CTLA-4 expression in lung adenocarcinoma and its TILs

①目的检测CTLA-4蛋白在肺腺癌细胞及其肿瘤浸润性淋巴细胞(TILs)中的表达,并探讨其与疾病进展的关系。②方法选取肺腺癌患者57例(观察组),良性肺病变患者20例(对照组)。采用新鲜标本冰冻切片后行免疫组化检测CTLA-4蛋白表达。③结果观察组肺腺癌细胞及其TILs中CTLA-4蛋白的阳性率均高于对照组(P<0.05);其中CTLA-4蛋白在肺腺癌细胞中的表达与分化程度有关,中-低分化组阳性率高于高分化组(P<0.05),而与性别、年龄、吸烟史、淋巴结转移及临床分期均无关(P>0.05)。CTLA-4蛋白在TILs中的表达与临床分期有关,Ⅲ-Ⅳ期组阳性率高于Ⅰ-Ⅱ组(P<0.05),而与性别、年龄、吸烟史、淋巴结转移及分化程度无关(P>0.05)。④结论CTLA-4蛋白在肺腺癌细胞及其TILs中的过表达可能参与肺腺癌的疾病进展。

Objective To detect the CTLA-4 protein expression in lung adenocarcinoma and its tumor infiltrating lymphocytes(TILs),and to explore the relationship between the CTLA-4 protein expression and the progression of lung adenocarcinoma.Methods A total of 57 lung adenocarcinoma (the observation group) and 20 benign lung disease (the control group) were selected.The CTLA-4 protein expression was detected by immunohistochemistry after frozen sections of fresh specimens.Results The positive rate of the CTLA-4 protein in lung adenocarcinoma cells and its TILs in the observation group was higher than that in the control group ( P < 0.05 ).Among them,the expression of the CTLA-4 protein in lung adenocarcinoma cells was related to the degree of differentiation,and the positive rate in the middle-low differentiation group was higher than that in the high differentiation group( P < 0.05 ),but was not related togender,age,smoking history,lymph node metastasis and the clinical stage( P > 0.05 ).The CTLA-4 protein expression in TILs was related to clinical stages,the positive rate in Ⅲ-Ⅳphase was higher than in theⅠ-Ⅱ group( P < 0.05 ),and had nothing to do with gender, age, smoking history, lymph node metastasis and the degree of differentiation( P > 0.05 ).Conclusion It is suggested that over-expression of the CTLA-4 protein in lung adenocarcinoma and its TILs may be involved in the disease progression of lung adenocarcinoma.