无机盐、激素与真菌联合诱导土沉香抗逆能力的研究

Resistance Capability of Aquilaria sinensis under Combinations of Inorganic Salts,Hormones and Fungus Induction

为了探究无机盐、激素与真菌联合诱导土沉香(Aquilaria sinensis)抗逆性及其与结香前期木芯变色长度之间的相关关系,以10年生土沉香为材料,采用均匀试验设计,开展了3种无机盐、3种激素及3种真菌组合试验。结果表明:(1)土沉香树体POD和SOD活性和MDA含量呈先升高后降低趋势,CAT活性在诱导后1天最高,随后呈下降趋势;(2)无机盐与真菌处理下土沉香的抗逆能力大于激素与真菌处理,其中无机盐与真菌组合试验的处理4(1.0%CaCl2+0.5%FeSO4+2.0%NaCl+黑绿木霉∶腐皮镰孢∶龙眼焦腐(1∶1∶1))和激素与真菌组合试验的处理2(0.01%茉莉酸甲酯+0.1%乙烯利+0.2%水杨酸钠+黑绿木霉∶腐皮镰孢∶龙眼焦腐(1∶1∶1))分别为同类处理最高;(3)土沉香抗逆能力高低与木芯变色长度存在显著正相关性;(4)理论上,土沉香抗逆能力最强诱导组合分别为0.93%CaCl2+0.53%FeSO4+2.5%NaCl+黑绿木霉∶腐皮镰孢∶龙眼焦腐(1∶1∶1)和0.005%茉莉酸甲酯+0.006%乙烯利+0.2%水杨酸钠+黑绿木霉∶腐皮镰孢∶龙眼焦腐(1∶1∶1)。

We explored the effect of different combinations of inorganic salts hormones and fungus induction on resistance capability of Aquilaria sinensis and the relationship between resistance and discoloration length of wood core in early stage of agarwood. Two induction experiments bydifferent combination of 3 kinds of inorganic salts,3 hormones and 3 fungi were carried out for ten-years-old trees of A.sinensis by uniform experimental design. The results showed that:(1)POD and SOD activity and MDA content firstly increased and then decreased;CAT activity reached the highest value at 1 day after induction,and then decreased;(2)The resistance capability under combinations of inorganic salts and fungus induction was greater than that of hormones and fungus. The capability in treatment 4(1.0% CaCl 2+0.5% FeSO 4+2.0% NaCl+ Trichoderma atroviride ∶ Fusarium solani ∶ Lasiodiplodia theobromae (1∶1∶1)) from combinations of inorganic salts and fungus and treatment 2(0.01% Methyl Jasmonate+0.1% Ethephon+0.2% Sodium salicylate+ Trichoderma atroviride ∶ Fusarium solani ∶ Lasiodiplodia theobromae (1∶1∶1)) from combinations of hormones and fungus was higher in the experiments respectively;(3)There was a significant positive correlation between the resistance capability of A.sinensis and the length of wood core discoloration;(4)Theoretically,0.93% CaCl 2+ 0.53% FeSO 4 +2.5% NaCl+ Trichoderma atroviride ∶ Fusarium solani ∶ Lasiodiplodia theobromae (1∶1∶1) and 0.005% Methyl Jasmonate+0.006% Ethephon+0.2% Sodium salicylate+ Trichoderma atroviride ∶ Fusarium solani ∶ Lasiodiplodia theobromae (1∶1∶1) was combination with the highest resistance ability respectively.