全基因组分析杨树WOX基因家族在茎部发育中的作用

Genome-Wide Analysis of the WOX Family Reveals Their Involvement in Stem Growth of Populus trichocarpa

WUSCHEL-related homeobox(WOX)家族是植物特有的转录因子家族,参与分生组织细胞分裂分化、初生和次生物质代谢及植物激素信号转导等多个发育过程,目前尚未有从全基因组分析该基因家族参与杨树茎部发育的相关研究。本项研究旨在对杨树WOX基因家族进行鉴定,在杨树基因中发现18个WOX候选基因,将这些候选基因分为三组,同一分组的大多数WOX家族成员具有相似的基因结构和保守的基序。根据不同发育阶段茎部转录组数据,系统分析了WOX家族成员在茎部不同发育阶段的特异表达情况,并采用qRT-PCR对上述结果进行了验证。结果表明,杨树WOX基因家族在茎部不同发育阶段表现出不同的表达模式,为毛果杨WOX家族的功能研究与利用奠定基础。

The WUSCHEL-related homeobox( WOX ) family is a plant-specific transcription factor family involved in multiple developmental processes including meristematic cell division and differentiation,primary and secondary biomass metabolism,and plant hormone signaling. At present,there is no relevant research on genome-wide analysis of the gene family involved in stem development of poplar. We identified the poplar WOX gene family,and found 18 WOX candidate genes in the poplar gene. These candidate genes were divided into three groups. Most of the WOX family members in the same group had similar gene structure and conservation motif. According to the transcriptome data of stems at different developmental stages,the specific expression of WOX family members in different developmental stages of stems was systematically analyzed,and the above results were verified by qRT-PCR. The poplar WOX gene family showed different expression patterns at different developmental stages of the stem,which laid a foundation for the functional research and utilization of the WOX family.