摘要
目的研究长链非编码RNA肺癌相关转录产物(LUCAT)1对宫颈癌细胞的活性、迁移和侵袭的影响,并探讨其机制。方法运用qRT-PCR法检测宫颈癌细胞Hela、正常宫颈细胞Ect1/E6E7中LUCAT1、miR-199a-5p的表达;将si-con组(转染si-con)、si-LUCAT1组(转染si-LUCAT1)、miR-199a-5p组(转染miR-199a-5p模拟物)、miR-NC组(转染miR-NC)、si-con+anti-miR-199a-5p组(共转染si-con和anti-miR-199a-5p)、si-LUCAT1+anti-miR-199a-5p组(共转染si-LUCAT1和anti-miR-199a-5p),均用脂质体法转染至Hela细胞;噻唑蓝(MTT)法检测各组细胞的活性;Transwell法检测各组细胞的迁移和侵袭;双荧光素酶报告基因检测实验检测各组细胞的荧光活性。结果与正常宫颈细胞Ect1/E6E7相比,宫颈癌细胞Hela中LUCAT1表达显著升高,miR-199a-5p表达显著降低;敲减LUCAT1、过表达miR-199a-5p均可明显抑制Hela细胞的活性、迁移和侵袭;miR-199a-5p可抑制野生型LUCAT1细胞的荧光活性,且LUCAT1可负向调控miR-199a-5p的表达;抑制miR-199a-5p可逆转敲减LUCAT1对Hela细胞的活性、迁移、侵袭的抑制作用。结论LUCAT1可促进宫颈癌细胞的活性、迁移、侵袭,其机制可能与靶向miR-199a-5p有关,将可为宫颈癌的治疗提供靶点。
Objective To study the effects of long-chain non-coding (Lnc) RNA lung cancer-associated transcript(LUCAT)1 on the activity,migration and invasion of cervical cancer cells and explore its mechanism. Methods qRT-PCR was used to detect the expression of LUCAT1 and miR-199a-5p in cervical cancer Hela and normal cervical cells Ect1/E6E7.si-con (si-con group),si-LUCAT1 (si-LUCAT1 group),miR-199a-5p mimics (miR-199a-5p group),miR-NC (miR-NC group),si-con and anti-miR-199a-5p (si-con+anti-miR-199a-5p group),si-LUCAT1+anti-miR-199a-5p (si-LUCAT1 and anti-miR-199a-5p group) were transfected into Hela cells by liposome respectively.The activity of each group was detected by MTT assay,the migration and invasion of each group was detected by transwell method,the fluorescence activity of each group was detected by dual luciferase reporter gene assay. Results Compared with normal cervical cells Ect1/E6E7,the expression of LUCAT1 in cervical cancer cells were significantly increased and the expression of miR-199a-5p were significantly decreased.Knockdown of LUCAT1 or overexpression of miR-199a-5p significantly inhibited the activity,migration and invasion of Hela cells;miR-199a-5p could inhibit the fluorescence activity of wild-type LUCAT1 cells and LUCAT1 could negatively regulate the expression of miR-199a-5p.Inhibition of miR-199a-5p could reverse the effect of the LUCAT1 knockdown on the inhibition of cell activity,migration and invasion of Hela cells. Conclusions Lnc RNA LUCAT1 could promote the activity,migration and invasion of cervical cancer cells.The mechanism might be related to targeting miR-199a-5p,which will provide a target for the treatment of cervical cancer.
作者
胡春艳
朱根海
邢艾文
崔开颖
HU Chun-Yan;ZHU Gen-Hai;XING Ai-Wen(Department of Gynaecology,Hainan General Hospital,Haikou 570311,Hainan,China)
出处
《中国老年学杂志》
CAS
北大核心
2019年第13期3257-3261,共5页
Chinese Journal of Gerontology
基金
海南省自然科学基金面上项目(No.20168279)