简单重复序列标记和序列相关扩增多态性标记在草莓遗传多样性分析中的比较

Comparison of simple sequence repeat(SSR) and sequence related amplified polymorphism(SRAP) markers for genetic diversity analysis in strawberry

利用简单重复序列(simple sequence repeat, SSR)和序列相关扩增多态性(sequence related amplifiedpolymorphism,SRAP)标记分析了来自国内外43个草莓品种的遗传多样性,并比较了这2种分子标记的效果差异。结果表明:30对SSR引物平均多态性位点数为6.43个,每个位点的平均多态性信息含量(polymorphisminformationcontent,PIC)值为0.6284;20个SRAP引物组合平均多态性位点数为14.30个,PIC值高达0.9114。基于SSR标记的聚类结果与基于SRAP标记的聚类结果的相关系数为0.817,呈显著水平。而SSR标记、SRAP标记分别与SSR+SRAP联合标记的相关系数为0.938和0.966,都达到极显著水平。SSR和SRAP标记都能较好地分析草莓遗传多样性,其中SRAP标记的效果略优于SSR标记,而SSR+SRAP联合标记分析则能更好地评估种质的遗传多样性和亲缘关系。

Simple sequence repeat (SSR) and sequence related amplified polymorphism (SRAP) markers were applied to analyze genetic diversity among 43 strawberry (Fragaria×ananassa Duch.) cultivars and effectivenesses of these two kinds of molecular markers were also compared. The results showed that there were 6.43 polymorphic sites per primer pair of 30 SSR primers, and the average polymorphism information content (PIC) of each site was 0.628 4. While there were 14.30 polymorphic sites per primer pair of 20 SRAP primers,and the average PIC of each site was 0.911 4. The correlation coefficient between clustering results based on SSR markers and SRAP markers was 0.817, which was significant. The correlation coefficients between SSR markers, SRAP markers and SSR+SRAP joint markers were 0.938 and 0.966, respectively, which reached extremely significant levels. Both SSR and SRAP markers can be used to analyze the genetic diversity of strawberry, but the effect of SRAP marker is better than that of SSR marker. The analysis of SSR+SRAP joint markers can better evaluate the genetic diversity and genetic relationship of strawberry germplasm.