茶叶中游离氨基酸总量的测量不确定度评定

Evaluation of the Measurement Uncertainty for Total Amount Determination of Free Amino Acids in Tea

通过对分光光度法测定茶叶中游离氨基酸总量的测量过程进行分析,根据《测量不确定度评定与表示》(JJF 1059.1-2012)中有关规定,识别检测过程的不确定度来源,并推导出不确定度计算公式,得出各种因素对检测结果的影响程度,发现标准曲线拟合对不确定度的影响最大,这为提高测量数据的质量提供了依据。

The spectrophotometric determination method was adopted to analyze the measurement for the determination of total amount of free amino acids in tea.According to the relevant regulations documented in the《Evaluation and Expression of Uncertainty in Measurement》(JJF 1059.1-2012),the source of the uncertainty in the detection processwas identified,and the uncertainty calculation formula was deduced.Comparing the different effects of various factors on the measure results,it was found that the standard curve fitting had the biggest impact on the uncertainty.This finding made contribution to improving the quality of data measurement.